Description

4-Nitrophenyl Phosphate Disodium Salt Hexahydrate is a chromogenic substrate widely used in enzymatic assays for the detection of alkaline phosphatase activity. Its high purity and consistent performance make it a critical reagent for ensuring accurate and reproducible results in diagnostic and research applications. This compound is essential for manufacturers and researchers in the fields of clinical diagnostics, life science research, and biochemical analysis.

Application

• Clinical Diagnostics: Primary substrate in colorimetric assays for alkaline phosphatase (ALP) in automated clinical chemistry analyzers for liver function and bone disorder panels.
• Life Science Research: Key reagent in ELISA (Enzyme-Linked Immunosorbent Assay) protocols and other immunoassays for detecting antibody or antigen presence.
• Biochemical Analysis: Used in enzyme kinetics studies to measure ALP activity and inhibition in purified enzyme preparations or cell lysates.
• Molecular Biology: Employed in nucleic acid detection systems where alkaline phosphatase is used as a reporter enzyme.
• Quality Control Reagent: Serves as a standard or control material in the production and validation of diagnostic kits and reagents.
• Pharmaceutical Testing: Utilized in drug discovery and development for screening compounds that modulate phosphatase activity.

Basic Information

Product Name	4-Nitrophenyl Phosphate Disodium Salt Hexahydrate
CAS No.	333338-18-4
Molecular Formula	C6H4NNa2O6P · 6H2O
Molecular Weight	371.14 g/mol (Anhydrous basis: 263.05 g/mol)
Synonyms	PNPP; p-Nitrophenyl phosphate disodium salt; 4-Nitrophenyl dihydrogen phosphate disodium salt hexahydrate; Disodium 4-nitrophenyl phosphate hexahydrate; Phosphoric acid 4-nitrophenyl ester disodium salt hexahydrate; ALP Substrate; Alkaline Phosphatase Substrate; Sigma P7998 (common catalog reference)
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl Phosphate Disodium Salt Hexahydrate is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, spectrophotometric analysis for absorbance characteristics, and water content determination, to ensure it meets the stringent requirements for diagnostic and research applications. Certificates of Analysis (COA) with full traceability are provided and available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store desiccated at the recommended temperature.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0% (on anhydrous basis)
Water Content (KF)	25.0% - 30.0% (Theoretical: 29.1%)
Absorbance (1% Solution, 405 nm)	≤ 0.05
Solubility (1M Diethanolamine Buffer)	Clear, colorless solution
Alkaline Phosphatase Activity Test	Suitable for use

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.