Description

[2-[2-[2-[2-[[(2,5-Dioxo-1-Pyrrolidinyl)Oxy]Carbonyl]-5-[3-(Trifluoromethyl)-3H-Diazirin-3-Yl]Phenoxy]Ethoxy]Ethoxy]Ethyl]-Carbamic Acid 1,1-Dimethylethyl Ester is a sophisticated heterobifunctional crosslinking agent designed for advanced bioconjugation and photoaffinity labeling applications. Its core value lies in the unique combination of a photoreactive trifluoromethyl-diazirine group and an amine-reactive NHS ester, enabling the covalent capture and study of transient biomolecular interactions. This compound is essential for researchers and developers in proteomics, chemical biology, and pharmaceutical discovery who require precise tools for mapping protein-protein interactions and identifying novel drug targets.

Application

• Photoaffinity Labeling (PAL): Used to covalently "trap" and identify low-affinity or transient protein-ligand and protein-protein interactions upon UV light activation.
• Chemical Proteomics: A key reagent for activity-based protein profiling (ABPP) to discover and characterize enzyme function and active sites in complex biological samples.
• Bioconjugation & Crosslinking: Facilitates the stable attachment of biomolecules (e.g., peptides, proteins, oligonucleotides) to surfaces, polymers, or other biomolecules for assay development and diagnostics.
• Drug Target Identification: Employed in the development of probe molecules to elucidate the binding sites and mechanisms of action for novel therapeutic candidates.
• Material Science Functionalization: Used to introduce photoreactive and bio-recognition groups onto nanomaterials, polymers, and biosensor surfaces.
• Structural Biology Studies: Aids in the study of protein structures and dynamics by introducing covalent crosslinks for analysis via mass spectrometry.

Basic Information

Product Name	[2-[2-[2-[2-[[(2,5-Dioxo-1-Pyrrolidinyl)Oxy]Carbonyl]-5-[3-(Trifluoromethyl)-3H-Diazirin-3-Yl]Phenoxy]Ethoxy]Ethoxy]Ethyl]-Carbamic Acid 1,1-Dimethylethyl Ester
CAS No.	332941-39-6
Molecular Formula	C25H31F3N4O9
Molecular Weight	588.53 g/mol
Synonyms	Boc-NH-PEG4-arylazide; N-(t-Boc)-amino-PEG4-arylazide; 3-(3-(Trifluoromethyl)-3H-diazirin-3-yl)-N-(2-(2-(2-(2-(3-((2,5-dioxopyrrolidin-1-yl)oxy)-3-oxopropoxy)ethoxy)ethoxy)ethyl)carbamoyl)propyl)benzamide (PEG4 spacer); t-Butyl N-[2-[2-[2-[2-[3-[3-[3-(trifluoromethyl)-3H-diazirin-3-yl]benzoyl]oxy]ethoxy]ethoxy]ethoxy]ethyl]carbamate; Boc-amine-PEG4-arylazide; Heterobifunctional Crosslinker (NHS ester/diazirine); Photoactivatable NHS-PEG4-Boc; Arylazide-PEG4-t-Boc
EINECS	Contact for details

Quality Control

Our production of this high-value biochemical reagent adheres to stringent quality protocols to ensure batch-to-batch consistency and optimal performance in sensitive research applications. Each lot undergoes comprehensive analytical characterization, including HPLC purity analysis, NMR, and mass spectrometry for structural confirmation. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting purity, identity, and functional suitability for photoaffinity labeling and conjugation workflows.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is strictly light-sensitive (protect from all light exposure) and hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Group Activity	Confirmed by assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.