Description

3-[(3-Cholamidopropyl)Dimethylammonio]-1-Propanesulfonate Hydrate 98% is a high-purity zwitterionic detergent widely recognized for its exceptional ability to solubilize membrane proteins while maintaining their native structure and function. This grade is critical for applications demanding high reproducibility and minimal interference in sensitive biochemical assays. It is an essential reagent for research and development laboratories in the biopharmaceutical, structural biology, and diagnostic sectors.

Application

• Membrane Protein Solubilization and Stabilization: Key for extracting and purifying integral membrane proteins for structural studies (e.g., X-ray crystallography, Cryo-EM) and functional assays.
• Cell Lysis Buffer Formulation: Used in gentle, non-denaturing lysis buffers to isolate active membrane-bound enzymes and receptors.
• Chromatography: Employed in the purification of membrane proteins via techniques such as affinity chromatography and size-exclusion chromatography.
• Diagnostic Kit Manufacturing: A component in buffers for immunoassays and other diagnostic tests that require stable protein conformations.
• Biopharmaceutical Development: Used in process development for the production and purification of therapeutic proteins, including antibodies and vaccines.
• Electrophoresis: Can be used in sample preparation for native PAGE or blue native PAGE to analyze protein complexes.

Basic Information

Product Name	3-[(3-Cholamidopropyl)Dimethylammonio]-1-Propanesulfonate Hydrate 98%
CAS No.	331717-45-4
Molecular Formula	C32H58N2O7S • xH2O
Molecular Weight	Anhydrous: 614.88 g/mol
Synonyms	CHAPS; 3-[(3-Cholamidopropyl)dimethylammonio]propanesulfonate; N,N-Dimethyl-3-sulfo-N-[3-(3α,7α,12α-trihydroxy-24-oxo-5β-cholan-24-yl)aminopropyl]propan-1-aminium hydroxide inner salt hydrate; Zwittergent 3-08; CHAPS Hydrate; 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate; N-[(3-Cholamidopropyl)dimethylammonio]butanesulfonate (common misnomer)
EINECS	Contact for details

Quality Control

Our 3-[(3-Cholamidopropyl)Dimethylammonio]-1-Propanesulfonate Hydrate 98% is manufactured under a quality management system and undergoes rigorous analytical testing to ensure batch-to-batch consistency and compliance with specifications critical for life science research. Each lot is supplied with a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance-related tests. We support compliance with relevant cGMP and ISO 9001 standards for research-use materials.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccant and under an inert atmosphere to maintain optimal purity and performance.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 5.0%
pH (1% Solution)	5.0 - 7.0
Critical Micelle Concentration (CMC)	6 - 10 mM (Typical)
Absorbance (280 nm, 1% Solution)	≤ 0.1
Heavy Metals (as Pb)	≤ 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.