Description

D(-)-Amethopterin Hydrate, 98 CAS NO 331717-43-2 is a high-purity, stereochemically defined derivative of the classic antifolate agent methotrexate. This specific enantiomer is of significant value in advanced pharmaceutical research and development, particularly for investigating structure-activity relationships and receptor-specific interactions. It is essential for researchers and manufacturers in the fields of oncology, immunology, and biochemistry who require precise chemical tools for drug discovery, biochemical assays, and the synthesis of targeted therapeutic agents.

Application

• Pharmaceutical Reference Standard: Serves as a critical certified reference material (CRM) for quality control and analytical method development in drug manufacturing.
• Biochemical Research: Used as a selective inhibitor of dihydrofolate reductase (DHFR) in enzymatic studies and metabolic pathway analysis.
• Anticancer Agent Development: Acts as a key intermediate or active pharmaceutical ingredient (API) precursor in the synthesis of novel chemotherapeutic compounds.
• Immunosuppressant Studies: Employed in research focused on modulating immune response for autoimmune disease and transplant rejection therapies.
• Academic & Institutional Research: Provides a high-purity reagent for university and institutional labs conducting life science and medicinal chemistry projects.
• Diagnostic Kit Manufacturing: Utilized in the formulation of diagnostic assays and kits that target folate-dependent pathways.

Basic Information

Product Name	D(-)-Amethopterin Hydrate, 98
CAS No.	331717-43-2
Molecular Formula	C₂₀H₂₂N₈O₅ • xH₂O
Molecular Weight	454.44 g/mol (anhydrous basis)
Synonyms	D-(-)-Methotrexate Hydrate; (2S)-2-[[4-[(2,4-Diaminopteridin-6-yl)methyl-methylamino]benzoyl]amino]pentanedioic acid hydrate; L-Glutamic acid, N-[4-[[(2,4-diamino-6-pteridinyl)methyl]methylamino]benzoyl]-, hydrate (1:1), (S)-; Amethopterin D-(-)-Form Hydrate; (S)-Methotrexate Hydrate; NSC-740; MTX Hydrate (D-form); Rheumatrex (stereospecific form)
EINECS	Contact for details

Quality Control

Every batch of our D(-)-Amethopterin Hydrate is produced and tested under a strict quality management system. We employ advanced analytical techniques including HPLC, NMR, and mass spectrometry to verify identity, purity, and chiral integrity. A comprehensive Certificate of Analysis (COA) detailing purity, chiral excess, residual solvents, and related substances is provided with each shipment to ensure it meets the exacting standards required for pharmaceutical research and development.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and must be kept in a desiccated environment to maintain stability and purity. For long-term storage, consider conditions under an inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0% (on anhydrous basis)
Chiral Purity (HPLC)	≥ 99.0% D(-)-enantiomer
Water Content (KF)	≤ 5.0% w/w
Residual Solvents (GC)	Complies with ICH Q3C guidelines
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.