Description

o-(6-Chlorobenzotriazol-1-Yl)-N,N,N',N'-Tetramethyluronium Tetrafluoroborate is a highly efficient peptide coupling reagent, commonly known as HCTU, widely used in solid-phase peptide synthesis (SPPS). This compound matters because it offers superior coupling efficiency with reduced racemization, making it a critical reagent for producing high-purity peptides and complex biomolecules. It is essential for pharmaceutical R&D, biotech companies, and academic laboratories engaged in the synthesis of therapeutic peptides, peptidomimetics, and other sensitive organic molecules.

Application

• Solid-Phase Peptide Synthesis (SPPS): Primary reagent for forming amide bonds between amino acids with minimal epimerization.
• Solution-Phase Peptide Synthesis: Used in the synthesis of peptides and small protein fragments in solution.
• Pharmaceutical Intermediate Production: Key building block in the manufacture of active pharmaceutical ingredients (APIs) for peptide-based drugs.
• Bioconjugation: Facilitates the coupling of peptides to other molecules, such as proteins, dyes, or polymers, for diagnostic and therapeutic applications.
• Combinatorial Chemistry: Employed in high-throughput synthesis for drug discovery libraries.
• Academic & Industrial Research: A staple reagent in laboratories for studying protein structure-function relationships and developing new bioactive compounds.

Basic Information

Item	Detail
Product Name	o-(6-Chlorobenzotriazol-1-Yl)-N,N,N',N'-Tetramethyluronium Tetrafluoroborate
CAS No.	330641-16-2
Molecular Formula	C13H16BClF4N6O
Molecular Weight	394.56 g/mol
Synonyms	HCTU; 1-[Bis(dimethylamino)methylene]-6-chloro-1H-benzotriazolium tetrafluoroborate; 6-Chloro-1H-benzotriazole-1-yloxy-tris(dimethylamino)phosphonium tetrafluoroborate (common misnomer); O-(6-Chloro-1H-benzotriazol-1-yl)-N,N,N',N'-tetramethyluronium tetrafluoroborate; Coupling Reagent HCTU; (6-Chloro-1H-benzotriazol-1-yloxy)tris(dimethylamino)phosphonium tetrafluoroborate; Tetramethyluronium Tetrafluoroborate HCTU
EINECS	Contact for details

Quality Control

Our o-(6-Chlorobenzotriazol-1-Yl)-N,N,N',N'-Tetramethyluronium Tetrafluoroborate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive synthetic applications. Certificates of Analysis (COA) with detailed specifications are available upon request to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and should be handled under dry conditions to maintain stability and performance. Keep the container tightly sealed in a desiccator after opening.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.