Description

Cytochrome P450 2D6 CAS NO 330597-62-1 is a highly purified, recombinant human enzyme belonging to the cytochrome P450 superfamily, a critical component in xenobiotic metabolism. This enzyme is essential for in vitro drug metabolism studies, enabling researchers to accurately predict and assess potential drug-drug interactions and metabolic pathways. It is primarily used by pharmaceutical R&D laboratories, contract research organizations (CROs), and academic institutions focused on pharmacology and toxicology.

Application

• Drug Metabolism and Pharmacokinetics (DMPK) Studies: For in vitro assessment of Phase I metabolism, specifically for substrates of the CYP2D6 isozyme.
• Drug-Drug Interaction (DDI) Screening: To identify and quantify inhibitory or inductive effects of new chemical entities on CYP2D6 activity, a key regulatory requirement.
• Enzyme Kinetics Characterization: Determination of Michaelis-Menten constants (Km, Vmax) for metabolic reactions catalyzed by CYP2D6.
• Metabolite Identification: Generation of CYP2D6-specific metabolites for structural elucidation using LC-MS or NMR.
• High-Throughput Screening (HTS): Integration into automated assay platforms for rapid screening of compound libraries against CYP2D6.
• Pharmacogenomics Research: Studying the functional impact of genetic polymorphisms (e.g., poor metabolizers vs. extensive metabolizers) on enzyme activity.
• Reaction Phenotyping: Determining the fraction of metabolism (fm) contributed by CYP2D6 for a given drug candidate.
• Biocatalysis: Potential use in the synthesis of specific chiral metabolites or pharmaceutical intermediates.

Basic Information

Product Name	Cytochrome P450 2D6
CAS No.	330597-62-1
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	CYP2D6; Cytochrome P450 Family 2 Subfamily D Member 6; Debrisoquine 4-Hydroxylase; Xenobiotic Monooxygenase 2D6; P450-DB; CYP2D; P450IID6; Recombinant Human CYP2D6; Cytochrome P450, Subfamily IID (Debrisoquine, Spartiene, etc., -Hydroxylating), Polypeptide 6
EINECS	Contact for details

Quality Control

Our recombinant Cytochrome P450 2D6 is produced under stringent conditions to ensure high specific activity and batch-to-batch consistency. Each lot undergoes comprehensive analytical testing, including activity assays with probe substrates (e.g., dextromethorphan, bufuralol), purity assessment via SDS-PAGE, and absence of contaminating P450 isoforms. Certificates of Analysis (COA) detailing purity, specific activity, concentration, and storage buffer composition are provided with every shipment to support your regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at ≤ -70°C in aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive). Upon thawing for use, keep on ice or at 2-8°C for short-term handling.

Specification

Item	Specification
Appearance	Clear solution
Concentration	≥ 1 nmol/mL (Contact for specific lot data)
Purity (SDS-PAGE)	≥ 90%
Specific Activity	≥ 3 nmol/min/nmol CYP (with dextromethorphan)
Reductase Activity	Fully coupled
Storage Buffer	50 mM Potassium Phosphate, pH 7.4, containing 20% (v/v) glycerol, 1 mM EDTA
Contaminating P450s	Not detected

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.