Description

Adenosine, n-Benzoyl-3'-Deoxy-3'-[[(4-Methoxyphenyl)Diphenylmethyl]Amino]-2'-o-Methyl-, 5'-[2-Cyanoethyl Bis(1-Methylethyl)Phosphoramidite] (9Ci) is a sophisticated, high-purity phosphoramidite monomer specifically engineered for oligonucleotide synthesis. This compound is critical for the automated solid-phase synthesis of DNA and RNA sequences, enabling the precise incorporation of modified nucleosides. It is an essential raw material for research institutions, diagnostic developers, and pharmaceutical companies working on antisense oligonucleotides, siRNA therapeutics, and molecular probes.

Application

• Oligonucleotide Synthesis: Primary building block (phosphoramidite) for the automated solid-phase synthesis of custom DNA and RNA strands.
• Therapeutic Development: Key intermediate in the production of modified antisense oligonucleotides and siRNA for targeted gene silencing therapies.
• Diagnostic Probe Manufacturing: Used to synthesize labeled probes for techniques like FISH (Fluorescence In Situ Hybridization) and PCR.
• Academic & Biotech Research: Enables the study of gene function, nucleic acid structure, and the development of novel bioassays.
• Synthesis of Aptamers: Facilitates the creation of structured nucleic acid ligands for binding specific target molecules.
• CRISPR/Cas9 Component Synthesis: Used in the preparation of guide RNAs (gRNAs) and other synthetic nucleic acid components for gene editing platforms.

Basic Information

Product Name	Adenosine, n-Benzoyl-3'-Deoxy-3'-[[(4-Methoxyphenyl)Diphenylmethyl]Amino]-2'-o-Methyl-, 5'-[2-Cyanoethyl Bis(1-Methylethyl)Phosphoramidite] (9Ci)
CAS No.	329773-22-0
Molecular Formula	C48H55N6O6P
Molecular Weight	843.0 g/mol
Synonyms	5'-O-(Bis(diisopropylamino)(2-cyanoethoxy)phosphino)-2'-O-methyl-3'-deoxy-3'-(N-(4-methoxytrityl)amino)-N-benzoyladenosine; 2'-O-Methyl-3'-deoxy-3'-(N-(4-methoxytrityl)amino)-N-benzoyladenosine-5'-[(2-cyanoethyl)-N,N-diisopropylphosphoramidite]; 2'-O-Me-3'-amino-3'-deoxy-N-bz-Adenosine CE Phosphoramidite; 2'-O-Methyl-3'-amino-3'-deoxy-N-benzoyladenosine phosphoramidite; 3'-Amino-2'-O-methyladenosine phosphoramidite (N-bz, MMTr protected); Amidite for 2'-O-Methyl, 3'-Amino modified Adenosine
EINECS	Contact for details

Quality Control

This high-value synthetic intermediate is produced under strict quality control protocols to ensure batch-to-batch consistency and optimal performance in automated synthesizers. Quality is verified by HPLC, 31P NMR, and 1H NMR to confirm identity, purity, and the absence of critical impurities like oxidated phosphite. A comprehensive Certificate of Analysis (COA) detailing purity, moisture content, and solvent residues is provided with each batch to support your quality management systems.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C ± 5°C under an inert atmosphere (argon or nitrogen) due to its moisture-sensitive and oxidizable nature. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (31P NMR)	Conforms
Purity (HPLC)	≥ 97.0%
Moisture (Karl Fischer)	≤ 0.5%
Solvent Residue (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.