Description

Cytidine 5'-(Tetrahydrogen Triphosphate), 5-Methyl- is a modified nucleoside triphosphate (NTP) analog, a critical building block for nucleic acid synthesis and research. This compound is essential for studying RNA structure, function, and epigenetic modifications, particularly those involving 5-methylcytidine. It is primarily utilized by biotechnology companies, academic research institutions, and pharmaceutical R&D departments engaged in enzymology, molecular biology, and the development of diagnostic assays or therapeutic oligonucleotides.

Application

• Enzymatic RNA Synthesis: As a substrate for RNA polymerases (e.g., T7 RNA polymerase) for the in vitro transcription of RNA containing 5-methylcytidine modifications.
• Epigenetics and RNA Modification Research: Fundamental tool for investigating the role and mechanism of 5-methylcytosine in RNA, relevant to gene expression regulation and cellular differentiation.
• Structural Biology Studies: Used to incorporate methylated bases into RNA for NMR or X-ray crystallography studies to understand RNA-protein interactions and folding.
• Diagnostic Probe Development: Synthesis of labeled or modified probes for detecting specific RNA sequences in diagnostic applications.
• Therapeutic Oligonucleotide R&D: Potential use in the research and development of modified antisense oligonucleotides (ASOs) or siRNA where enhanced stability or specific binding is required.
• Biocatalysis and Enzyme Mechanism Studies: Serves as a specific substrate to study the kinetics and specificity of methyltransferases and other nucleic acid-processing enzymes.

Basic Information

Product Name	Cytidine 5'-(Tetrahydrogen Triphosphate), 5-Methyl-
CAS No.	327174-86-7
Molecular Formula	C10H18N3O14P3
Molecular Weight	521.17 g/mol
Synonyms	5-Methyl-CTP; 5-Methylcytidine-5'-triphosphate; 5-Methylcytidine triphosphate; m5CTP; 5-Methylcytidine 5'-triphosphate; 4-Amino-5-methyl-1-β-D-ribofuranosyl-2(1H)-pyrimidinone 5'-triphosphate
EINECS	Contact for details

Quality Control

Our Cytidine 5'-(Tetrahydrogen Triphosphate), 5-Methyl- is produced under stringent quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive biochemical applications. Certificates of Analysis (COA) detailing purity, impurity profile, and other critical parameters are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (Karl Fischer)	≤ 5.0%
pH (1% Solution)	6.0 - 8.0
Biological Activity	Suitable for enzymatic incorporation (e.g., in vitro transcription)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.