Description

Dmtr-Tna-C(Bz)-Amidite CAS NO 325683-96-3 is a specialized phosphoramidite monomer used in the automated solid-phase synthesis of oligonucleotides. This compound is critical for the precise and efficient incorporation of modified nucleosides into DNA and RNA sequences, enabling advanced research and therapeutic development. It is an essential raw material for biotechnology, pharmaceutical R&D, and diagnostic kit manufacturers focused on creating high-purity, sequence-specific nucleic acid products.

Application

• Oligonucleotide Synthesis: As a key building block (amidite) in automated DNA/RNA synthesizers for the production of antisense oligonucleotides, primers, and probes.
• Therapeutic Development: For the synthesis of modified oligonucleotides used in siRNA, aptamer, and antisense drug discovery programs.
• Diagnostic Assays: Manufacture of fluorescently labeled or otherwise modified probes for PCR, qPCR, FISH, and microarray applications.
• Research Reagents: Production of custom oligonucleotides for academic and industrial research in genomics, molecular biology, and biochemistry.
• Next-Generation Sequencing (NGS): Synthesis of specialized adapters, barcodes, and primers used in NGS library preparation workflows.
• CRISPR/Cas9 Systems: Synthesis of guide RNAs (gRNAs) with specific chemical modifications to enhance stability and editing efficiency.

Basic Information

Product Name	Dmtr-Tna-C(Bz)-Amidite
CAS No.	325683-96-3
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-O-(tert-butyldimethylsilyl)-N4-benzoyl-3'-O-(2-cyanoethyl-N,N-diisopropylphosphoramidite)cytidine; DMT-T-C(Bz)-amidite (2'-TBDMS); Cytidine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2'-O-[[(1,1-dimethylethyl)dimethylsilyl]-N4-benzoyl-3'-O-[2-[[bis(1-methylethyl)amino](2-cyanoethoxy)phosphino]ethyl]-; Amidite monomer for C (Cytidine) with DMT and TBDMS protecting groups; N4-Benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-O-(tert-butyldimethylsilyl)cytidine 3'-O-(2-cyanoethyl N,N-diisopropylphosphoramidite)
EINECS	Contact for details

Quality Control

Our Dmtr-Tna-C(Bz)-Amidite is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and 31P NMR and 1H NMR for structural confirmation, to ensure it meets the high standards required for oligonucleotide synthesis. Certificates of Analysis (COA) with detailed specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below under an inert atmosphere (argon or nitrogen) due to its hygroscopic and moisture-sensitive nature. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms
Identification (31P NMR)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.