Description

Sulfo-NHS-SS-Biotin is a heterobifunctional, water-soluble, and cleavable crosslinking reagent designed for bioconjugation. This compound is critical for introducing biotin tags onto proteins, antibodies, and other biomolecules via amine groups, enabling subsequent capture or detection with streptavidin. It is an essential tool for researchers and developers in proteomics, immunoassay development, and targeted drug delivery systems. The cleavable disulfide (SS) linker allows for the gentle elution of captured targets under reducing conditions.

Application

• Protein and Antibody Biotinylation: For labeling primary amines (lysine residues) to create probes for ELISA, Western blotting, and flow cytometry.
• Affinity Chromatography Ligand Immobilization: Conjugation to resins or surfaces for capturing streptavidin/avidin-tagged biomolecules with reversible elution.
• Cell Surface Labeling: Tagging membrane proteins on live cells for isolation, tracking, or pull-down assays.
• Diagnostic Assay Development: Creating sensitive detection systems in lateral flow tests and clinical diagnostic kits.
• Drug Conjugate & ADC Research: Facilitating the development of cleavable linker systems in biopharmaceuticals.
• Biomolecular Interaction Studies: Used in pull-down assays and mass spectrometry (MS) sample preparation for protein complex analysis.

Basic Information

Product Name	Sulfo-NHS-SS-Biotin
CAS No.	325143-98-4
Molecular Formula	C20H30N4O9S3
Molecular Weight	606.68 g/mol
Synonyms	Sulfo-N-hydroxysuccinimide-SS-biotin; NHS-SS-Biotin (sulfonated); Biotin-HPDP; Sulfosuccinimidyl 2-(biotinamido)ethyl-1,3-dithiopropionate; EZ-Link Sulfo-NHS-SS-Biotin; N-(6-(Biotinamido)hexyl)-3'-(2'-pyridyldithio)propionamide sulfonate; Biotinylation reagent, cleavable; Water-soluble NHS-SS-Biotin
EINECS	Contact for details

Quality Control

Our Sulfo-NHS-SS-Biotin is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and functional activity validation. Certificates of Analysis (COA) with detailed specifications are provided to ensure compliance with research and development standards. We support traceability and consistency for critical applications in pharmaceutical and diagnostic development.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term storage, keep under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMSO
Functional Activity	Conforms (Biotinylation assay)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.