Description

Tfp-Peo-Biotin* CAS NO 318511-01-2 is a specialized heterobifunctional crosslinking reagent that combines a tetrafluorophenyl (TFP) ester, a polyethylene oxide (PEO) spacer, and a biotin moiety. This compound is engineered for the efficient, site-specific biotinylation of proteins, peptides, and other amine-containing biomolecules under mild aqueous conditions. It is an essential tool for researchers and manufacturers in bioconjugation, diagnostic assay development, and targeted drug delivery systems, where controlled labeling and subsequent purification or detection via streptavidin are required.

Application

• Bioconjugation and Protein Labeling: Site-specific attachment of biotin to antibodies, enzymes, and other proteins for use in immunoassays (ELISA, Western Blot) and biosensors.
• Affinity Purification Probes: Creation of biotin-tagged ligands, peptides, or small molecules for pull-down assays and streptavidin-based purification workflows.
• Diagnostic Kit Development: Key reagent for constructing sensitive detection systems in clinical diagnostics and point-of-care testing devices.
• Drug Delivery & Targeting: Functionalization of nanoparticles, liposomes, or polymer carriers with biotin for targeted therapeutic applications.
• Cell Surface Engineering: Labeling of cell surface amines for tracking, sorting, or studying cell-matrix interactions.
• Microarray and Chip Fabrication: Immobilization of biomolecules onto solid supports in a oriented manner for high-throughput screening.
• Structural Biology: Preparation of biotinylated samples for techniques like cryo-electron microscopy using streptavidin-gold conjugates.

Basic Information

Product Name	Tfp-Peo-Biotin*
CAS No.	318511-01-2
Molecular Formula	C₃₀H₃₃F₄N₅O₈S (Contact for exact confirmation)
Molecular Weight	~699.7 g/mol (Contact for exact confirmation)
Synonyms	N-(Biotinoyl)-N'-(PEO4-2,3,5,6-tetrafluorophenyl)succinamate; TFP-PEG4-Biotin; 2,3,5,6-Tetrafluorophenyl 3-(2-(2-(2-(2-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)ethoxy)ethoxy)ethoxy)ethoxy)propanoate; Tetrafluorophenyl-PEO4-Biotin; Biotin-PEO4-TFP Ester; Succinimidyl-[(biotinamido)tetraethyleneglycol] 2,3,5,6-tetrafluorophenyl ester; Heterobifunctional Crosslinker TFP-PEO-Biotin
EINECS	Contact for details

Quality Control

Every batch of Tfp-Peo-Biotin* is manufactured and analyzed under strict quality protocols to ensure high purity and consistent performance. Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) are available upon request, providing detailed results for purity (HPLC), identity (NMR, MS), and functional integrity.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Activity	Conforms (Biotin binding & amine reactivity)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.