Description

2-Naphthyl-β-D-Galactopyranoside is a specialized chromogenic and fluorogenic substrate used in enzymatic assays. This compound is critical for researchers and manufacturers requiring precise detection and quantification of β-galactosidase activity. It is primarily utilized in molecular biology, diagnostic kit development, and high-throughput screening within the pharmaceutical and biotechnology sectors.

Application

• Enzymatic Substrate for β-Galactosidase: Used as a chromogenic or fluorogenic substrate in assays to detect and quantify β-galactosidase (lacZ) activity in molecular biology and microbiology.
• Reporter Gene Assays: Essential in research involving lacZ reporter gene systems for studying gene expression and regulation.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits and reagents for clinical and research laboratories.
• High-Throughput Screening (HTS): Employed in pharmaceutical and biotech drug discovery platforms to identify enzyme inhibitors or activators.
• Cell & Tissue Staining: Used in histochemistry and cytochemistry for visualizing β-galactosidase expression in cells and tissues, including applications in senescence detection.
• Biochemical Research: Serves as a tool compound for enzyme kinetics studies and mechanistic investigations of glycosidase enzymes.

Basic Information

Product Name	2-Naphthyl-β-D-Galactopyranoside
CAS No.	312693-81-5
Molecular Formula	C16H18O6
Molecular Weight	306.31 g/mol
Synonyms	2-Naphthyl β-D-galactopyranoside; β-D-Galactopyranoside, 2-naphthyl; 2-Naphthol β-D-galactoside; 6-(2-Naphthyl)-β-D-galactopyranose; o-Nitrophenyl-β-D-galactopyranoside (common analog); ONPG analog; Chromogenic galactoside substrate; Fluorogenic galactosidase substrate
EINECS	Contact for details

Quality Control

Every batch of 2-Naphthyl-β-D-Galactopyranoside is manufactured and tested under a strict quality management system. We ensure high purity and batch-to-batch consistency suitable for sensitive research and diagnostic applications. Comprehensive analysis includes identity confirmation, purity assay, and impurity profiling. A Certificate of Analysis (COA) documenting all test results is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to minimize exposure to atmospheric humidity. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Melting Point	Contact for details
Specific Rotation	Contact for details
Heavy Metals	< 10 ppm
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.