Description

Arg-Glu(Edans)-(Asn670,Leu671)-App770 (668-675)-Lys(Dabcyl)-Arg is a specialized, high-purity peptide substrate designed for fluorescence resonance energy transfer (FRET) assays. This compound matters as a critical tool for researchers studying protease activity, particularly in the context of Alzheimer's disease research and related neurological pathways. It is primarily needed by pharmaceutical R&D laboratories, diagnostic kit manufacturers, and academic institutions focused on enzymology and neurodegenerative disease mechanisms.

Application

• Protease Activity Assays: Specifically designed as a FRET-based substrate for monitoring β-secretase (BACE-1) activity, a key enzyme in amyloid precursor protein (APP) processing.
• Alzheimer's Disease Research: Enables the screening and evaluation of potential therapeutic inhibitors targeting the amyloidogenic pathway.
• High-Throughput Screening (HTS): Used in automated drug discovery platforms to identify novel compounds that modulate secretase enzymes.
• Enzyme Kinetic Studies: Facilitates the determination of kinetic parameters (Km, Vmax) for β-secretase and related proteases.
• Diagnostic Development: Serves as a core component in the development of in vitro diagnostic tests for enzymatic biomarkers.
• Biochemical Research: A vital reagent for studying protein cleavage, signal transduction, and peptide metabolism in cellular models.

Basic Information

Product Name	Arg-Glu(Edans)-(Asn670,Leu671)-App770 (668-675)-Lys(Dabcyl)-Arg
CAS No.	310427-94-2
Molecular Formula	C84H118N22O26S2
Molecular Weight	1884.1 g/mol
Synonyms	BACE-1 FRET Substrate; APP770 (668-675) FRET Peptide; β-Secretase Substrate I; Edans/Dabcyl-Labeled APP Peptide; SEVNLDAEFK(Edans/Dabcyl)RR; Fluorogenic BACE-1 Substrate; Alzheimer's Disease FRET Peptide; Amyloid Precursor Protein Fragment (668-675)
EINECS	Contact for details

Quality Control

Our Arg-Glu(Edans)-(Asn670,Leu671)-App770 (668-675)-Lys(Dabcyl)-Arg is manufactured under strict quality protocols to ensure batch-to-batch consistency and optimal performance in sensitive assays. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional activity. We adhere to ISO 9001 quality management standards, and our production environment is suitable for research-grade biochemicals.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive). For long-term storage, desiccate. Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC & MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Peptide Content	≥ 70%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Functional Activity	Cleavage by BACE-1 produces a measurable FRET signal change

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.