Description

n-Biotinyl-N'-(3-Maleimidopropionyl)-3,6-Dioxaoctane-1,8-Diamine is a heterobifunctional crosslinking reagent designed for the site-specific conjugation of biomolecules. This compound matters because it combines the high-affinity biotin-streptavidin interaction with a maleimide group for thiol-selective coupling, enabling precise labeling and immobilization strategies. It is primarily needed by researchers and manufacturers in bioconjugation, diagnostic assay development, and targeted drug delivery systems who require reliable and efficient linker chemistry.

Application

• Bioconjugation and Protein Labeling: Site-specific attachment of biotin tags to antibodies, enzymes, or other proteins via cysteine residues for detection and purification.
• Diagnostic Assay Development: Fabrication of biosensors and immunoassay components by immobilizing capture probes onto streptavidin-coated surfaces.
• Affinity Chromatography: Synthesis of biotinylated ligands for capture on streptavidin or avidin resins.
• Targeted Drug Delivery: Conjugation of therapeutic agents to targeting moieties like antibodies or peptides.
• Cell Surface Engineering: Labeling of cell surface thiols for tracking, sorting, or functional studies.
• Nanoparticle Functionalization: Modification of nanoparticles for biomedical imaging and therapeutic applications.
• Microarray Technology: Immobilization of biomolecules in a defined orientation on chip surfaces.

Basic Information

Product Name	n-Biotinyl-N'-(3-Maleimidopropionyl)-3,6-Dioxaoctane-1,8-Diamine
CAS No.	305372-39-8
Molecular Formula	C24H39N5O7S
Molecular Weight	541.66 g/mol
Synonyms	Biotin-PEG3-Maleimide; Biotin-PEG3-Mal; Maleimide-PEG3-Biotin; 3,6,9-Trioxa-12-azatetradecanedioic acid, 14-[(5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoyl)amino]-4,13-dioxo-10-(2,5-dihydro-2,5-dioxo-1H-pyrrol-1-yl)-, 1,14-diester; Biotinamido-PEG3-Maleimide; Biotin-PEG3-Maleimide Crosslinker; EZ-Link Biotin-PEG3-Maleimide; Biotin-(PEG)3-Maleimide
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards for research and bioconjugation applications. Every batch is characterized by HPLC, NMR, and mass spectrometry to confirm identity, purity, and functional integrity. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.