Description

Propyl-Agarose is a functionalized agarose chromatography medium, chemically modified by the covalent attachment of propyl groups to the polysaccharide backbone. This modification creates a hydrophobic interaction chromatography (HIC) matrix, a critical tool for the purification of biomolecules based on surface hydrophobicity. It is essential for researchers and process development scientists in the biotechnology and pharmaceutical industries who require high-resolution separation of proteins, antibodies, and other complex biologicals under mild, non-denaturing conditions.

Application

• Hydrophobic Interaction Chromatography (HIC) for the purification of proteins, peptides, and monoclonal antibodies.
• Intermediate Purification Step in multi-column downstream processing workflows for biologics.
• Separation of Biomolecule Variants such as glycoforms, aggregates, or clipped species based on subtle hydrophobic differences.
• Virus Purification and vaccine development processes.
• Enzyme and Recombinant Protein Purification in both research and Good Manufacturing Practice (GMP) environments.
• Analytical Method Development for characterizing the hydrophobicity profile of therapeutic proteins.

Basic Information

Product Name	Propyl-Agarose
CAS No.	39347-14-3
Molecular Formula	(C12H18O7)n (Base agarose structure with propyl substituents)
Molecular Weight	Polymeric material (Contact for details)
Synonyms	Agarose propyl ether; Propyl agarose; n-Propyl agarose; Agarose, 2-hydroxypropyl ether; Hydrophobic agarose; HIC agarose; Propyl-Sepharose (common trade name style); Agarose-based HIC medium
EINECS	Contact for details

Quality Control

Our Propyl-Agarose is manufactured under a quality management system and undergoes rigorous testing to ensure batch-to-batch consistency for chromatographic performance. Key parameters such as ligand density, bead size distribution, flow properties, and pressure tolerance are strictly controlled. A Certificate of Analysis (COA) detailing these specifications is provided with each batch to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. The product is supplied as a suspension in a preservative solution (e.g., 20% ethanol); do not allow the matrix to dry out. Keep away from strong alkalis and organic solvents.

Specification

Item	Specification
Matrix	Cross-linked 6% Agarose
Ligand	n-Propyl
Ligand Density	~20-40 μmol/mL gel (Typical)
Bead Size	45-165 μm (wet)
Maximum Flow Rate	>300 cm/h (at 20°C)
pH Stability	3 - 13 (long term); 2 - 14 (short term)
Chemical Stability	Stable in all commonly used aqueous buffers, detergents, and 8M urea. Avoid strong oxidizing agents.
Storage Solution	20% Ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.