Description

L-Glutamate Oxidase is a highly specific enzyme that catalyzes the oxidative deamination of L-glutamic acid, producing α-ketoglutarate, ammonia, and hydrogen peroxide. This specificity makes it an indispensable biocatalyst and analytical reagent for the selective detection and quantification of L-glutamate. It is essential for industries and research institutions requiring precise enzymatic analysis, biosensor development, and biochemical process control.

Application

• Biosensor & Diagnostic Kits: Core component in amperometric and colorimetric biosensors for glutamate monitoring in food, clinical samples (blood, cerebrospinal fluid), and fermentation broths.
• Food & Beverage Analysis: Used in enzymatic assay kits to determine free glutamate content, a key umami flavor indicator, in products like sauces, soups, and processed foods.
• Pharmaceutical & Neurochemical Research: Critical tool for studying glutamatergic neurotransmission, screening neuroactive compounds, and quantifying glutamate in cell culture and tissue samples.
• Fermentation Process Monitoring: Enables real-time, specific measurement of L-glutamate concentration during microbial fermentation for amino acid production (e.g., monosodium glutamate).
• Clinical Diagnostics: Employed in automated analyzers for the detection of glutamate in physiological fluids, aiding in the diagnosis and research of neurological disorders.
• Enzyme-Based Reagent Formulation: Serves as a key ingredient in the formulation of stable, ready-to-use enzymatic reagents for high-throughput analyzers.

Basic Information

Item	Detail
Product Name	L-Glutamate Oxidase
CAS No.	39346-34-4
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	L-Glutamate Oxidase; Glutamate Oxidase; L-Glutamic Oxidase; L-Glutamate:Oxygen Oxidoreductase (deaminating); EC 1.4.3.11; GOx; GluOx
EINECS	Contact for details

Quality Control

Our L-Glutamate Oxidase is produced and tested under stringent quality management systems. Each batch undergoes comprehensive analysis to ensure high specific activity, purity, and stability. Key parameters including activity (U/mg), protein content, and absence of interfering enzyme activities are verified. A Certificate of Analysis (COA) detailing all test results is provided with every shipment to guarantee traceability and compliance with your application requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. The product is hygroscopic (moisture-sensitive). For long-term stability, consider storage at -20°C under desiccated conditions. Allow the product to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Specific Activity	≥ 10 U/mg protein
Protein Content (Biuret)	Contact for details
Purity (SDS-PAGE)	≥ 90%
Stabilizers	May contain buffer salts (e.g., phosphate) and stabilizers (e.g., BSA, sucrose)
Solubility	Soluble in buffer (e.g., 10-50 mM phosphate buffer, pH 7.0)
Optimum pH	7.0 - 8.0
Optimum Temperature	30 - 37°C
Inhibitors	Heavy metal ions, sulfhydryl reagents

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.