Description

p-Nitrophenyl β-D-Galactopyranosiduronic Acid is a chromogenic substrate specifically designed for the detection and quantification of β-glucuronidase enzyme activity. This compound is critical for research and diagnostic applications where precise enzymatic measurement is required. It is primarily utilized by biotechnology, pharmaceutical, and clinical diagnostics industries for enzyme-linked assays, microbial identification, and high-throughput screening.

Application

• Enzymatic Substrate for β-Glucuronidase: Used as a specific chromogenic substrate in biochemical assays to detect and measure β-glucuronidase activity, producing a yellow-colored p-nitrophenol upon hydrolysis.
• Microbiological Testing: Employed in microbial identification systems, particularly for differentiating Escherichia coli and other enteric bacteria based on their glucuronidase activity.
• Diagnostic Reagent Manufacturing: A key raw material in the production of diagnostic kits and reagents for clinical laboratories.
• Pharmaceutical Research & Development: Used in drug discovery and development for screening enzyme inhibitors and studying drug metabolism pathways involving glucuronidation.
• Food Safety and Water Quality Testing: Applied in rapid detection methods for fecal coliforms and E. coli contamination in food and water samples.
• Cell Biology and Reporter Gene Assays: Utilized in molecular and cellular biology research as a substrate in reporter gene systems to study gene expression and regulation.

Basic Information

Product Name	p-Nitrophenyl β-D-Galactopyranosiduronic Acid
CAS No.	39031-76-0
Molecular Formula	C12H13NO9
Molecular Weight	315.23 g/mol
Synonyms	PNPG; p-Nitrophenyl β-D-Glucuronide; 4-Nitrophenyl β-D-Glucopyranosiduronic Acid; p-Nitrophenyl β-D-Glucuronide; β-Glucuronidase Substrate; 4-Nitrophenyl β-D-Glucuronide; pNPG; 1-O-(4-Nitrophenyl)-β-D-glucopyranuronic acid
EINECS	Contact for details

Quality Control

Our p-Nitrophenyl β-D-Galactopyranosiduronic Acid is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency suitable for sensitive diagnostic and research applications. Each lot is tested to meet stringent in-house specifications. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the container to reach room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Melting Point	Contact for details
Specific Rotation	Contact for details
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.