Description

4-Nitrophenyl-A-D-Galacturonide is a chromogenic substrate specifically designed for the enzymatic detection and quantification of α-galacturonidase activity. This compound is critical for researchers and quality control laboratories requiring precise, colorimetric assays in biochemical and industrial processes. It is primarily utilized in the food science, plant biology, and biotechnology sectors for analyzing pectin degradation, enzyme characterization, and diagnostic test development.

Application

• Enzyme Activity Assays: Primary substrate for the spectrophotometric determination of α-galacturonidase (EC 3.2.1.67) activity in research and industrial enzyme preparations.
• Food & Beverage Industry: Used in quality control to monitor pectinase activity during fruit juice clarification, wine making, and other processes involving pectin degradation.
• Plant Pathology & Physiology: Employed in research to study plant-microbe interactions, fruit ripening, and cell wall metabolism by measuring endogenous enzyme levels.
• Diagnostic Development: Serves as a key reagent in developing diagnostic kits for the detection of specific microbial pathogens or genetic traits based on enzyme activity.
• Biotechnology & Enzyme Production: Essential for screening microbial strains, optimizing fermentation conditions, and standardizing enzyme products in biotech manufacturing.
• Academic & Pharmaceutical Research: Utilized in biochemical studies to investigate enzyme kinetics, inhibition, and mechanism of action for potential therapeutic targets.

Basic Information

Product Name	4-Nitrophenyl-A-D-Galacturonide
CAS No.	39031-75-9
Molecular Formula	C₁₂H₁₃NO₉
Molecular Weight	315.23 g/mol
Synonyms	p-Nitrophenyl α-D-galactopyranosiduronic acid; 4-Nitrophenyl α-D-galacturonide; PNPG; pNP-α-D-GalA; α-D-Galacturonic acid 4-nitrophenyl ester; 4-Nitrophenyl-α-D-galactopyranosiduronic acid; 4-Nitrophenyl α-D-galactopyranuronoside
EINECS	Contact for details

Quality Control

Every batch of 4-Nitrophenyl-A-D-Galacturonide is manufactured and tested under strict quality management protocols to ensure high purity and consistent performance in sensitive enzymatic assays. Our products undergo rigorous quality testing, including HPLC for purity verification, to ensure compliance with industry standards. Certificates of Analysis (COA) with detailed chromatographic data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry, cool place. The product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to ambient humidity or light may affect stability and assay performance.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Specific Rotation	Contact for details
Solubility	Soluble in water, DMSO
Absorbance (Lambda max)	~400 nm (upon enzymatic cleavage)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.