Description

4'-Methylumbelliferyl-2-Acetamido-3,4,6-Tri-Acetyl-2-Deoxy-β-D-Glucopyranoside is a specialized fluorogenic substrate designed for the sensitive detection and quantification of N-acetyl-β-D-glucosaminidase (NAGase) activity. This compound matters significantly in biochemical research and diagnostics, as the enzymatic cleavage releases the highly fluorescent 4-methylumbelliferone, enabling precise, real-time kinetic assays. It is primarily needed by pharmaceutical R&D teams, clinical diagnostic laboratories, and academic researchers focused on lysosomal storage disorders, renal function studies, and high-throughput enzyme inhibitor screening.

Application

• Enzymatic Assay Substrate: A critical fluorogenic substrate for the specific and sensitive detection of N-acetyl-β-D-glucosaminidase (NAGase) activity in vitro.
• Diagnostic Reagent Manufacturing: Used in the formulation of diagnostic kits for monitoring renal tubular damage, where urinary NAGase is a key biomarker.
• Biochemical Research: Essential for studying enzyme kinetics, mechanism of action, and inhibition profiles in lysosomal biology and glycobiology research.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) campaigns to identify and characterize potential therapeutic inhibitors for lysosomal storage diseases like Tay-Sachs and Sandhoff disease.
• Quality Control in Bioprocessing: Used to assay for contaminating glycosidase activity in the production of therapeutic proteins and other biologics.

Basic Information

Product Name	4'-Methylumbelliferyl-2-Acetamido-3,4,6-Tri-Acetyl-2-Deoxy-β-D-Glucopyranoside
CAS No.	38971-29-8
Molecular Formula	C24H27NO13
Molecular Weight	537.47 g/mol
Synonyms	4-MU-β-GlcNAc-3,4,6-triacetate; 4-Methylumbelliferyl N-acetyl-β-D-glucosaminide triacetate; 4-Methylumbelliferyl 2-acetamido-2-deoxy-β-D-glucopyranoside-3,4,6-triacetate; MUG-NAG-3,4,6-TriAc; 4-MU-β-GlcNAc(Ac)3; Fluorogenic NAGase substrate; NAGase substrate, fluorogenic; 2-Acetamido-1-N-(4-methyl-2-oxo-2H-chromen-7-yl)-2-deoxy-3,4,6-tri-O-acetyl-β-D-glucopyranoside
EINECS	Contact for details

Quality Control

Our 4'-Methylumbelliferyl-2-Acetamido-3,4,6-Tri-Acetyl-2-Deoxy-β-D-Glucopyranoside is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency, critical for reliable assay performance. Each lot undergoes comprehensive analytical testing including HPLC for purity, NMR for structural confirmation, and functional enzymatic assay verification. Certificates of Analysis (COA) detailing all specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines
Functional Assay (Enzymatic)	≥ 95% of theoretical activity

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.