Description

5-Bromo-4-Chloro-3-Indolylphosphate is a chromogenic phosphatase substrate widely used in molecular biology and diagnostic applications. This compound matters for its critical role as a key reagent in enzyme-linked immunoassays and blotting techniques, enabling the sensitive and specific detection of target proteins or nucleic acids. It is primarily needed by manufacturers of diagnostic kits, research institutions, and biotechnology companies engaged in life science research and development.

Application

• As a key chromogenic substrate for alkaline phosphatase (AP) in Western blotting (BCIP/NBT system).
• For the detection of nucleic acid hybridization in Southern and Northern blotting procedures.
• In the development and production of enzyme-linked immunosorbent assay (ELISA) kits and other immunoassays.
• As a critical component in histochemical staining for in situ hybridization and immunohistochemistry.
• For research applications in cell biology and microbiology to visualize phosphatase activity.
• In the quality control of biopharmaceuticals for impurity detection and characterization.

Basic Information

Product Name	5-Bromo-4-Chloro-3-Indolylphosphate
CAS No.	38404-93-2
Molecular Formula	C8H6BrClNO4P
Molecular Weight	326.47 g/mol
Synonyms	BCIP; 5-Bromo-4-chloro-3-indolyl phosphate; 4-Chloro-5-bromo-3-indolyl phosphate; BCIP disodium salt; 3-Indolylphosphate, 5-bromo-4-chloro-; X-Phosphate; 5-Bromo-4-chloro-1H-indol-3-yl dihydrogen phosphate
EINECS	Contact for details

Quality Control

Our 5-Bromo-4-Chloro-3-Indolylphosphate is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency suitable for sensitive diagnostic and research applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing all specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMF, DMSO
Heavy Metals	< 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.