Description

Dimethyl 3,3'-Dithio-Bis(Propionimidate) Dihydrochloride is a high-purity, bifunctional crosslinking reagent primarily used for the covalent modification of proteins and other biomolecules. Its core value lies in its ability to introduce stable, cleavable disulfide bridges between primary amines, a critical function in bioconjugation and structural biology research. This compound is essential for researchers and manufacturers in the fields of biopharmaceutical development, diagnostic assay production, and advanced materials science.

Application

• Protein Crosslinking and Conjugation: A key reagent for creating antibody-drug conjugates (ADCs), enzyme complexes, and immobilized protein matrices.
• Biomolecule Immobilization: Used to covalently attach proteins, peptides, or antibodies to solid supports for biosensors, diagnostic chips, and affinity chromatography resins.
• Structural Analysis: Facilitates the study of protein-protein interactions and quaternary structure by introducing reversible crosslinks for mass spectrometry (MS) analysis.
• Polymer Modification: Serves as a functional linker for synthesizing smart polymers and hydrogels with cleavable, responsive properties for drug delivery systems.
• Diagnostic Reagent Synthesis: Employed in the development of stable, high-sensitivity reagents for immunoassays (ELISA, CLIA) and lateral flow tests.
• Cell Surface Labeling: Used to tag cell surface proteins for tracking, sorting, or studying membrane dynamics via flow cytometry or microscopy.

Basic Information

Product Name	Dimethyl 3,3'-Dithio-Bis(Propionimidate) Dihydrochloride
CAS No.	38285-78-8
Molecular Formula	C8H16N2O2S2 · 2HCl
Molecular Weight	329.28 g/mol
Synonyms	DTBP; Dimethyl 3,3'-dithiobispropionimidate dihydrochloride; 3,3'-Dithiobis(N,N-dimethylpropionimidamide) dihydrochloride; Bis(2-imidoyl ethyl) disulfide dihydrochloride; DTBP Crosslinker; DTME (Dimethyl 3,3'-dithiobispropionimidate) dihydrochloride; Dithiobispropionimidate ester dihydrochloride; DTBP·2HCl
EINECS	Contact for details

Quality Control

Our Dimethyl 3,3'-Dithio-Bis(Propionimidate) Dihydrochloride is manufactured under strict quality management systems to ensure batch-to-batch consistency and reliability for sensitive research and production applications. Each lot undergoes comprehensive analytical testing, including HPLC purity analysis, NMR structural confirmation, and functional activity validation. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting purity, identity, and relevant physical characteristics.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and performance. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in water, DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.