Description

Lambda Exonuclease is a highly specific enzyme that catalyzes the processive 5' to 3' digestion of double-stranded DNA, selectively removing nucleotides from 5'-phosphorylated termini. This precise activity makes it an indispensable tool for molecular biology applications requiring the removal of one strand of DNA. It is a critical reagent for researchers and developers in the fields of genetic engineering, next-generation sequencing (NGS) library preparation, and recombinant DNA technology. Its reliable performance is essential for ensuring the accuracy and efficiency of downstream analytical processes.

Application

• Next-Generation Sequencing (NGS): Preparation of ssDNA templates for sequencing platforms by digesting the phosphorylated strand of PCR products.
• Site-Directed Mutagenesis: Generation of single-stranded DNA templates for oligonucleotide-based mutagenesis protocols.
• DNA-Protein Interaction Studies: Creating substrates for electrophoretic mobility shift assays (EMSAs) or footprinting analyses.
• Recombinant DNA Technology: Digesting vector backbones or PCR products to facilitate specific cloning strategies and reduce background.
• Structural Biology: Preparing defined DNA substrates for crystallography or other biophysical characterization methods.
• Diagnostic Assay Development: Used in the creation of sensitive detection systems that rely on strand-specific digestion.

Basic Information

Product Name	Lambda Exonuclease
CAS No.	37367-70-7
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Exodeoxyribonuclease lambda; 5'→3'-exonuclease; Bacteriophage lambda exonuclease; λ Exonuclease; EC 3.1.11.3; Lambda (λ) Exo; 5'-Phosphomonoester-producing exodeoxyribonuclease
EINECS	Contact for details

Quality Control

Our Lambda Exonuclease is produced under stringent quality management systems. Each lot undergoes rigorous functional activity assays, purity assessments, and contamination checks (including tests for DNase, RNase, and protease activities) to ensure consistent performance and lot-to-lot reproducibility. Certificates of Analysis (COA) detailing specific activity, purity, and buffer composition are available upon request to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C. The product is supplied in a buffered glycerol solution. Avoid repeated freeze-thaw cycles. Keep the container tightly sealed to prevent moisture absorption and contamination.

Specification

Item	Specification
Appearance	Clear, colorless solution
Identification (Activity)	Functional activity confirmed
Specific Activity	> 5,000 units/mg protein
Purity (SDS-PAGE)	> 95%
Contaminating Nuclease Activity	Not detected
Contaminating Protease Activity	Not detected
Storage Buffer	50 mM Tris-HCl, pH 8.0, 50% Glycerol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.