Description

Phosphoenolpyruvate Carboxylase is a critical biocatalyst that irreversibly fixes carbon dioxide onto phosphoenolpyruvate (PEP) to form oxaloacetate. This enzymatic activity is fundamental to primary metabolism in plants and bacteria, making it an essential reagent for biochemical research and industrial bioprocessing. It is primarily utilized by researchers in plant physiology, enzymology, and metabolic engineering, as well as by manufacturers in the biotechnology sector for diagnostic kit production and biosynthetic pathway development.

Application

• Biochemical Research & Assay Development: A key reagent for studying C4 and CAM plant photosynthesis, anaplerotic reactions, and metabolic flux analysis.
• Diagnostic Kit Manufacturing: Used as a core enzyme component in clinical and research diagnostic kits for measuring metabolites like PEP, CO2, or related enzymatic activities.
• Enzymatic Synthesis: Employed in vitro for the biocatalytic production of oxaloacetate and other four-carbon dicarboxylic acids for fine chemical synthesis.
• Metabolic Engineering: Serves as a tool enzyme for reconstructing and optimizing synthetic pathways in microbial cell factories for the production of biofuels and biochemicals.
• Educational & Teaching Kits: Supplied for university-level laboratory courses in biochemistry and molecular biology to demonstrate enzyme kinetics and carbon fixation mechanisms.
• Agricultural Biotechnology: Used in research aimed at enhancing crop yield and stress tolerance by manipulating photosynthetic efficiency.

Basic Information

Product Name	Phosphoenolpyruvate Carboxylase
CAS No.	37341-55-2
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	PEP Carboxylase; PEPCase; PEPC; Phosphoenolpyruvate carboxylase; Phosphopyruvate carboxylase; Orthophosphate:oxaloacetate carboxy-lyase (phosphorylating); EC 4.1.1.31
EINECS	Contact for details

Quality Control

Our Phosphoenolpyruvate Carboxylase is produced and tested under stringent quality management systems. Each lot is characterized for specific activity, purity, and stability to ensure consistent performance in sensitive applications. A comprehensive Certificate of Analysis (COA) detailing assay results, protein concentration, and activity units is provided with every shipment. We adhere to relevant guidelines for enzyme production to support research and industrial scale requirements.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below in a non-frost-free freezer. The product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Conforms
Specific Activity	≥ 10 units/mg protein
Protein Content (Biuret)	Contact for details
Purity (SDS-PAGE)	≥ 90%
Stabilizers	May contain sucrose or glycerol
pH (in solution)	7.0 - 8.0

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.