Description

Lyticase is a highly purified enzyme complex derived from *Arthrobacter luteus*, specifically engineered for the efficient lysis of yeast cell walls. This product is essential for researchers and industrial bioprocessors requiring high-yield extraction of intracellular components, such as proteins, nucleic acids, and organelles, while maintaining their integrity. It is a critical reagent in molecular biology, pharmaceutical development, and biotechnology for applications ranging from recombinant protein production to the preparation of yeast spheroplasts.

Application

• Yeast Cell Wall Lysis: Primary application for the preparation of spheroplasts and protoplasts in *Saccharomyces cerevisiae* and other yeast species.
• Protein Extraction: Efficient release of intracellular recombinant proteins and enzymes from yeast expression systems for downstream purification.
• Genomic DNA Preparation: Facilitates the isolation of high-molecular-weight genomic DNA from yeast cells for PCR, sequencing, and cloning.
• Mitochondria Isolation: Used in protocols for the gentle disruption of yeast cells to isolate intact, functional mitochondria for metabolic studies.
• Bioprocessing: Employed in industrial fermentation and biocatalysis processes to disrupt yeast biomass and recover valuable metabolites.
• Diagnostic Kit Component: Serves as a key enzymatic component in commercial kits for yeast identification and fungal infection diagnostics.

Basic Information

Product Name	Lyticase
CAS No.	37340-57-1
Molecular Formula	N/A (Enzyme Complex)
Molecular Weight	N/A (Enzyme Complex)
Synonyms	β-1,3-Glucanase; Zymolyase; Yeast Lytic Enzyme; 1,3-β-Glucan 3-Glucanohydrolase; Arthrobacter Luteus Lytic Enzyme; Glucan Endo-1,3-β-D-Glucosidase; Endo-1,3(4)-β-Glucanase; CAS 37340-57-1
EINECS	253-485-8

Quality Control

Our Lyticase is produced under strict quality management systems. Each lot is tested to ensure high specific activity, purity, and consistency. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing parameters such as enzymatic activity (units/mg), protein content, and absence of contaminating protease or nuclease activities. We support compliance with relevant guidelines for research and industrial use.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This product is hygroscopic (moisture-sensitive). For long-term stability, consider storage at -20°C. Keep the container sealed in a dry environment to prevent loss of activity.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (IR)	Conforms to reference standard
Specific Activity	≥ 20,000 units/mg protein
Protein Content (Biuret)	≥ 90%
Protease Activity	≤ 0.1 units/mg
RNase & DNase Activity	Not detected
Solubility	Clear, slightly opalescent solution in water or buffer
pH (1% solution)	6.0 - 7.5

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.