Description

Methyl-Agarose CAS NO 37328-76-0 is a chemically modified polysaccharide derived from agarose, where hydroxyl groups are partially substituted with methoxy groups. This modification significantly enhances its hydrophobic interaction properties, making it a superior matrix for the purification of biomolecules. It is an essential tool for research and industrial laboratories in the biotechnology, pharmaceutical, and life sciences sectors, particularly for hydrophobic interaction chromatography (HIC).

Application

• Hydrophobic Interaction Chromatography (HIC): Primary matrix for the separation and purification of proteins, enzymes, and antibodies based on surface hydrophobicity.
• Biopharmaceutical Downstream Processing: Critical step in purifying monoclonal antibodies, recombinant proteins, and other therapeutic biomolecules.
• Enzyme Purification: Isolation of lipases, dehydrogenases, and other enzymes that benefit from HIC techniques.
• Nucleic Acid Research: Used in specialized protocols for the separation of nucleic acid-binding proteins or complexes.
• Diagnostic Kit Manufacturing: Component in purification columns for in-vitro diagnostic (IVD) reagents.
• Vaccine Development: Aids in the purification of viral antigens and other vaccine components.

Basic Information

Product Name	Methyl-Agarose
CAS No.	37328-76-0
Molecular Formula	(C7H12O5)n
Molecular Weight	Polymeric (Variable)
Synonyms	Agarose Methyl Ether; Methyl Agarose; Methoxy Agarose; Agarose, methyl ether; Hydrophobic Agarose; HIC Agarose; 6-O-Methylagarose; Agarose O-methyl ether
EINECS	Contact for details

Quality Control

Our Methyl-Agarose is manufactured under a strict quality management system. Each batch is tested to ensure consistent performance in chromatographic applications, with key parameters including ligand substitution degree, bead size distribution, and flow properties. Certificates of Analysis (COA) detailing purity and functional specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to prevent moisture uptake, which can affect performance.

Specification

Item	Specification
Appearance	White to off-white beads
Identification (IR)	Conforms to structure
Ligand Substitution (Degree of Methylation)	Contact for details
Bead Size	45-165 μm (or as specified)
Exclusion Limit	> 2 x 107 Daltons
pH Stability (Working Range)	3 - 12
Maximum Operating Pressure	0.3 MPa (3 bar, 44 psi)
Storage Solution	20% Ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.