Description

Heparinase I F. Flavobacterium Heparinum ~1 U/Mg, 100 Ug** is a highly purified, specific lyase enzyme derived from *Flavobacterium heparinum*. This product is critical for the controlled depolymerization and structural analysis of heparin and heparan sulfate glycosaminoglycans. It is an essential tool for researchers and manufacturers in the biopharmaceutical and diagnostic industries requiring precise enzymatic degradation. The enzyme's defined activity of approximately 1 unit per milligram ensures reliable and reproducible results in analytical and process applications.

Application

• Heparin/Heparan Sulfate Structural Analysis: Used to cleave glycosidic linkages for sequencing and determining sulfation patterns.
• Low Molecular Weight Heparin (LMWH) Production: Employed in the controlled enzymatic manufacturing process of LMWH therapeutics.
• Pharmaceutical Quality Control: Essential for testing heparin-based drug purity, potency, and for detecting contaminants like oversulfated chondroitin sulfate (OSCS).
• Biochemical Research: A key reagent for studying glycosaminoglycan-protein interactions, cell signaling, and developmental biology.
• Diagnostic Kit Development: Incorporated into assays for measuring heparin levels or activity in clinical samples.
• Glycobiology Studies: Used to prepare defined oligosaccharide fragments from heparin and heparan sulfate for functional studies.

Basic Information

Item	Detail
Product Name	Heparinase I F. Flavobacterium Heparinum ~1 U/Mg, 100 Ug**
CAS No.	37290-85-0
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Heparinase I; Heparin Lyase I; Heparin eliminase; Flavobacterium heparinum Heparinase I; EC 4.2.2.7; Heparinase (Flavobacterium heparinum); Glycosaminoglycan lyase I
EINECS	Contact for details

Quality Control

Our Heparinase I is produced and tested under stringent conditions to ensure high purity, specific activity, and lot-to-lot consistency suitable for research and pharmaceutical applications. Each batch is characterized for enzymatic activity, protein content, and purity profile. A comprehensive Certificate of Analysis (COA) detailing assay results, sterility (where applicable), and stability data is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation. For extended use, aliquot the product to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Specific Activity	~1 U/mg protein (vs. Heparin Sodium)
Protein Content (Biuret)	>90%
Identification (Activity)	Conforms
Purity (SDS-PAGE)	Single major band
Endotoxin	< 1.0 EU/μg (where specified)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.