Description

Alpha1-2 Fucosidase is a highly specific glycoside hydrolase enzyme that catalyzes the hydrolysis of terminal α1-2 linked fucose residues from glycoproteins and glycolipids. This precise enzymatic activity is critical for structural analysis and modification of complex carbohydrates, enabling research into cell signaling, pathogen recognition, and biomarker discovery. It is an essential tool for biotechnology, pharmaceutical development, and advanced life science research applications requiring detailed glycan profiling.

Application

• Glycobiology Research: Used for the structural elucidation and sequencing of N-linked and O-linked glycans by selectively removing α1-2 linked fucose.
• Biopharmaceutical Analysis: Critical for characterizing the glycosylation patterns of therapeutic monoclonal antibodies (mAbs) and other recombinant proteins to ensure consistency and efficacy.
• Diagnostic Development: Employed in the development of assays for detecting fucosylation-related disease biomarkers, including certain cancers and inflammatory conditions.
• Enzyme-Assisted Synthesis: Utilized in the chemoenzymatic synthesis of defined oligosaccharides and glycoconjugates for research and potential therapeutic use.
• Blood Group Antigen Modification: Applied in studies of ABO and Lewis blood group antigens, which contain α1-2 fucosylated structures.
• Microbiome & Host-Pathogen Interaction Studies: Helps analyze fucosylated glycans on gut epithelial cells and their role in microbial adhesion and symbiosis.

Basic Information

Product Name	Alpha1-2 Fucosidase
CAS No.	37288-45-2
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	α1-2 Fucosidase; 1,2-α-L-Fucosidase; α-L-Fucosidase (1→2); α-L-Fucosidase (EC 3.2.1.51); Glycosidase F1; Fucosidase I; Exo-α-sialidase (occasional misnomer); Blood Group Substance degrading enzyme
EINECS	Contact for details

Quality Control

Our Alpha1-2 Fucosidase is produced and tested under stringent quality management systems. Each lot is characterized for specific activity, purity, and absence of contaminating glycosidase activities. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing specifications for activity (U/mg), protein purity (by SDS-PAGE), and buffer composition. Our quality protocols are designed to support research and development requiring high reproducibility and performance.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C or below. Avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after use to prevent absorption of atmospheric moisture. Store in a cool, dry, and well-ventilated area.

Specification

Item	Specification
Appearance	Clear to slightly opalescent solution
Identification (Activity)	Hydrolyzes α1-2 linked fucose specifically
Specific Activity	≥ XX U/mg protein
Purity (SDS-PAGE)	≥ 90%
Protein Concentration	XX mg/mL
Buffer	XX mM Phosphate, pH X.X
Stabilizers	Glycerol, BSA (optional)
Contaminating Activities	Negligible exo/endo-glycosidase activity

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.