Description

Mung Bean Nuclease is a single-strand specific endonuclease derived from mung bean sprouts, widely recognized for its high specificity in cleaving single-stranded DNA and RNA. This enzyme is a critical tool in molecular biology for the removal of single-stranded overhangs from double-stranded DNA, enabling precise nucleic acid manipulation. It is essential for researchers and manufacturers in biotechnology, pharmaceutical development, and molecular diagnostics who require high-purity enzymatic reagents for cloning, sequencing, and hybridization protection assays.

Application

• Removal of single-stranded overhangs from double-stranded DNA to create blunt ends for cloning.
• Mapping mutations and analyzing nucleic acid structure in molecular biology research.
• Cleaving hairpin loops and other single-stranded regions in PCR products or synthesized oligonucleotides.
• Preparation of templates for DNA sequencing by eliminating ssDNA contaminants.
• Use in nuclease protection assays (e.g., S1 mapping) for mRNA analysis and quantification.
• Processing of recombinant DNA in the production of biologics and gene therapies.
• Quality control applications in the synthesis of primers and probes for diagnostic kits.

Basic Information

Product Name	Mung Bean Nuclease
CAS No.	37288-25-8
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Mung Bean Nuclease I; Nuclease S1 (Aspergillus oryzae) analog; Single-Strand-Specific Nuclease; EC 3.1.30.1; Endonuclease S1; Single-Stranded Nucleic Acid Endonuclease; MBN
EINECS	Contact for details

Quality Control

Our Mung Bean Nuclease is produced and tested under stringent quality management systems to ensure batch-to-batch consistency and high enzymatic activity. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, specific activity, and absence of contaminating nucleases. We adhere to relevant quality standards suitable for research and in-vitro diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. The product is hygroscopic (moisture-sensitive) and should be kept under desiccated conditions. Aliquot upon receipt to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or solution
Identification (Activity)	Conforms
Specific Activity	> 100,000 units/mg protein
Purity (SDS-PAGE)	> 95%
Double-Stranded DNA Degradation	< 1%
RNase Contamination	Not detected
Protease Contamination	Not detected
pH Optimum	4.5 - 5.5

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.