Description

Ribonuclease T2 From Aspergillus Oryzae is a highly specific endoribonuclease enzyme that catalyzes the hydrolysis of phosphodiester bonds in RNA, producing nucleoside 3'-phosphates and oligonucleotides with terminal 3'-phosphates. This specificity makes it an indispensable tool for RNA sequence analysis and structural studies. It is primarily utilized by research institutions, diagnostic kit manufacturers, and biotechnology companies engaged in molecular biology, genomics, and nucleic acid research.

Application

• RNA Sequencing and Fingerprinting: Used to digest RNA for generating sequence-specific fragments.
• Structural Biology Research: Employed in probing RNA secondary and tertiary structure.
• Diagnostic Reagent Manufacturing: A key component in enzymatic assay kits for RNA detection and analysis.
• Biochemical Research: Essential for studying RNA metabolism, degradation pathways, and enzyme kinetics.
• Pharmaceutical Development: Used in quality control and research for RNA-based therapeutics and vaccines.

Basic Information

Product Name	Ribonuclease T2 From Aspergillus Oryzae
CAS No.	37278-25-4
Molecular Formula	N/A (Protein)
Molecular Weight	Approx. 36 kDa (Monomer)
Synonyms	RNase T2; Ribonuclease T2; Aspergillus oryzae Ribonuclease; Guanyloribonuclease; Endoribonuclease T2; Acid Ribonuclease; EC 3.1.27.1; Ribonucleate 3'-pyrimidino-oligonucleotidohydrolase
EINECS	Contact for details

Quality Control

Our Ribonuclease T2 is produced and tested under stringent quality management systems. Each batch is characterized for specific activity, purity, and absence of contaminating nucleases. Certificates of Analysis (COA) detailing assay results, including activity units/mg protein, protein concentration, and purity by SDS-PAGE, are provided to ensure compliance with research-grade specifications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized powder at -20°C. Reconstituted enzyme solutions should be aliquoted and stored at -20°C or below to prevent loss of activity. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (Activity)	Conforms (Hydrolyzes RNA)
Specific Activity	≥ 50,000 units/mg protein
Purity (SDS-PAGE)	≥ 90%
Protein Content (Biuret)	≥ 70%
Contaminating Nucleases	Not detected
Solubility	Soluble in water or buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.