Description

Dna Ligase CAS NO 37259-52-2 is a critical enzyme that catalyzes the formation of a phosphodiester bond between the 5'-phosphate and 3'-hydroxyl termini of DNA strands, a fundamental process for DNA replication and repair. This high-purity enzyme is essential for ensuring the integrity and efficiency of molecular biology workflows, directly impacting research outcomes and biotechnological production. It is a vital reagent for molecular biology laboratories, pharmaceutical R&D, and biotechnology companies engaged in genetic engineering, cloning, and next-generation sequencing applications.

Application

• Molecular Cloning: Essential for joining DNA fragments with compatible ends in plasmid vector construction.
• Next-Generation Sequencing (NGS) Library Preparation: Used to ligate adapters to DNA fragments for sequencing platforms.
• DNA Repair and Recombination Studies: A key reagent in enzymatic assays to study DNA repair pathways and homologous recombination.
• Site-Directed Mutagenesis: Facilitates the circularization of oligonucleotide-incorporated DNA strands for creating specific mutations.
• Diagnostic Assay Development: Employed in the development of ligase chain reaction (LCR) and other amplification-based diagnostic tools.
• Synthetic Biology: Critical for the assembly of large DNA constructs and genetic circuits from standardized biological parts (BioBricks).

Basic Information

Product Name	Dna Ligase
CAS No.	37259-52-2
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	DNA Ligase; Polydeoxyribonucleotide synthase (ATP); EC 6.5.1.1; T4 DNA Ligase (common source); ATP-dependent DNA ligase; DNA joinase; Polynucleotide ligase; Deoxyribonucleic ligase; DNA repair enzyme; Ligation enzyme
EINECS	Contact for details

Quality Control

Our Dna Ligase is produced and tested under stringent quality management systems to ensure batch-to-batch consistency and optimal activity. Each lot is subjected to rigorous analytical testing, including activity assays, purity assessment, and tests for contaminating nucleases. A comprehensive Certificate of Analysis (COA) detailing purity, specific activity, and storage buffer composition is provided with every shipment to support your quality and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C in a non-frost-free freezer. The product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature before opening to prevent condensation. Aliquotting upon receipt is recommended for frequent use to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Clear, colorless solution
Identification (Activity)	Conforms
Specific Activity	≥ 200,000 units/mg protein
Purity (SDS-PAGE)	≥ 95%
Endonuclease Activity	Not detectable
Exonuclease Activity	Not detectable
pH	7.5 - 8.0
Storage Buffer	10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.