Description

3-Oxoacyl-(Acyl-Carrier-Protein) Reductase is a critical enzyme involved in the fatty acid biosynthesis pathway, specifically catalyzing the reduction of a 3-oxoacyl-(acyl-carrier-protein) to a (3R)-3-hydroxyacyl-(acyl-carrier-protein). This product is essential for biochemical research and development, particularly in the study of metabolic pathways and enzyme mechanisms. It is primarily utilized by research institutions, pharmaceutical R&D departments, and biotechnology companies focused on metabolic engineering, antibiotic discovery, and lipid metabolism studies.

Application

• Biochemical Research: Fundamental studies on the fatty acid synthase (FAS) system and type II fatty acid biosynthesis pathways.
• Drug Discovery & Target Validation: Screening and validation of novel antibacterial agents that target bacterial fatty acid synthesis, a crucial process for bacterial survival.
• Metabolic Engineering: Used in synthetic biology applications to engineer microbial strains for the production of specialized fatty acids, biofuels, or other valuable compounds.
• Enzyme Kinetics & Mechanism Studies: Investigation of catalytic activity, substrate specificity, and inhibition kinetics of FabG (its common name in bacteria).
• Diagnostic Kit Development: A component in enzymatic assays designed to measure specific metabolites or pathway activities in clinical or agricultural samples.
• Academic Teaching: Serves as a model enzyme for graduate and postgraduate level courses in biochemistry, enzymology, and microbiology.

Basic Information

Product Name	3-Oxoacyl-(Acyl-Carrier-Protein) Reductase
CAS No.	37250-34-3
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	3-Oxoacyl-[acyl-carrier-protein] reductase; FabG; β-ketoacyl-acyl carrier protein reductase; 3-Oxoacyl-ACP reductase; EC 1.1.1.100; (3R)-3-Hydroxyacyl-[acyl-carrier-protein] dehydrogenase; NADPH-dependent 3-oxoacyl-[acyl-carrier-protein] reductase
EINECS	Contact for details

Quality Control

Our 3-Oxoacyl-(Acyl-Carrier-Protein) Reductase is produced and handled under stringent quality management protocols. Each batch undergoes rigorous analytical testing to ensure high purity, specific activity, and functional integrity. Quality assurance includes assessment of protein concentration, enzymatic activity, and the absence of contaminating proteases or nucleases. A comprehensive Certificate of Analysis (COA) detailing purity, activity units, buffer composition, and storage conditions is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below in aliquots to avoid repeated freeze-thaw cycles. The product is typically supplied in a buffered glycerol solution. Handle using standard sterile techniques for enzymatic reagents to maintain activity and prevent microbial contamination.

Specification

Item	Specification
Appearance	Clear, colorless to slightly yellow solution
Protein Concentration	≥ 1 mg/mL (by Bradford or UV absorbance)
Specific Activity	≥ XX units/mg protein (Contact for details)
Purity (SDS-PAGE)	≥ 90%
Buffer	XX mM Tris-HCl, pH X.X, XX% (v/v) glycerol
Stabilizers/Additives	None or DTT (as specified)
Contaminants	Protease activity: Not detected; Nuclease activity: Not detected

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.