Description

T4 Polynucleotide Kinase is a highly specific enzyme that catalyzes the transfer of the γ-phosphate from ATP to the 5'-hydroxyl terminus of DNA or RNA. This product is essential for molecular biology research, enabling critical techniques in genetic analysis and manipulation. It is a fundamental reagent for molecular biologists, geneticists, and researchers in pharmaceutical and diagnostic development working with nucleic acids. CAS No. 37211-65-7.

Application

This enzyme is indispensable in a wide range of molecular biology protocols, including:

• 5'-End Labeling of Nucleic Acids: For preparing probes for hybridization, sequencing, and microarray analysis.
• Linker/Adapter Phosphorylation: A critical step in cloning procedures to ensure efficient ligation.
• Site-Directed Mutagenesis: Used to phosphorylate oligonucleotides for subsequent ligation steps in mutagenesis kits.
• Next-Generation Sequencing (NGS) Library Preparation: For phosphorylating DNA fragments to facilitate adapter ligation.
• DNA/RNA Footprinting: In the preparation of end-labeled DNA fragments for structural studies.
• Restriction Enzyme Mapping: To generate labeled DNA fragments for mapping studies.
• Biochemical Research: Studies involving DNA repair mechanisms, recombination, and other kinase-related pathways.

Basic Information

Product Name	T4 Polynucleotide Kinase
CAS No.	37211-65-7
Molecular Formula	N/A (Enzyme)
Molecular Weight	Approx. 33 kDa (monomer)
Synonyms	T4 PNK; Polynucleotide 5'-Hydroxyl-Kinase; ATP:5'-dephosphopolynucleotide 5'-phosphotransferase; EC 2.7.1.78; T4 Bacteriophage Polynucleotide Kinase; PNK; T4 Polynucleotide 5'-Hydroxyl-Kinase
EINECS	Contact for details

Quality Control

Our T4 Polynucleotide Kinase is produced under stringent quality systems. Each lot is tested for optimal enzymatic activity, purity, and the absence of contaminating nucleases and phosphatases to ensure reliable and reproducible results in sensitive applications. A comprehensive Certificate of Analysis (COA) detailing activity units, purity, and specific storage buffer composition is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C in a non-frost-free freezer. The product is supplied in a buffered glycerol solution to prevent freezing at -20°C. Avoid repeated freeze-thaw cycles. The enzyme is hygroscopic (moisture-sensitive); ensure the vial is tightly sealed after use.

Specification

Item	Specification
Appearance	Clear, colorless solution
Activity	≥ 10,000 units/mL
Purity (SDS-PAGE)	≥ 95%
Exonuclease Activity	Not detectable
Endonuclease Activity	Not detectable
Phosphatase Activity	Not detectable
Storage Buffer	50 mM Tris-HCl, 5 mM DTT, 50% Glycerol (v/v)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.