Description

Nuclease, Ribo- (Purine-Specific) is a highly specific endonuclease enzyme that catalyzes the cleavage of RNA at purine bases. This specificity makes it an indispensable tool for RNA mapping, structural analysis, and the removal of RNA contamination from DNA preparations. It is essential for molecular biology research, pharmaceutical development, and diagnostic applications where precise RNA manipulation is required.

Application

• RNA Sequence Analysis: Used for ribonuclease protection assays (RPA) and RNA fingerprinting to map RNA structure and sequence.
• DNA Purification: Critical for the removal of contaminating RNA from genomic DNA and plasmid preparations to ensure purity for downstream applications like PCR and sequencing.
• Molecular Biology Research: A key reagent in studies of RNA metabolism, gene expression analysis, and the characterization of ribonucleoprotein complexes.
• Pharmaceutical Development: Employed in the quality control of RNA-based therapeutics and vaccines to analyze integrity and confirm sequence-specific cleavage.
• Diagnostic Kit Manufacturing: Integrated into diagnostic kits for pathogen detection (e.g., viral RNA) that require specific RNA processing steps.
• Biochemical Studies: Used to investigate enzyme kinetics, RNA-protein interactions, and the mechanisms of nucleic acid degradation.

Basic Information

Product Name	Nuclease, Ribo- (Purine-Specific)
CAS No.	37205-57-5
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Ribonuclease T1; RNase T1; Guanyloribonuclease; Endoribonuclease T1; Ribonuclease Guanine-specific; Aspergillus oryzae Ribonuclease; RNase N1; Ribonuclease N1
EINECS	Contact for details

Quality Control

Our Nuclease, Ribo- (Purine-Specific) is produced and tested under stringent quality management systems. Each batch is characterized for specific activity, purity, and the absence of contaminating nucleases (e.g., DNase, non-specific RNase) and proteases. Certificates of Analysis (COA) detailing assay results, storage buffer composition, and activity units are provided with every shipment to ensure traceability and compliance with research and production standards.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C in aliquots to avoid repeated freeze-thaw cycles. The enzyme is supplied in a buffered glycerol solution; ensure it does not freeze completely at -20°C. Handle with appropriate laboratory precautions.

Specification

Item	Specification
Appearance	Clear, colorless solution
Identification (Activity)	Cleaves RNA specifically at guanosine residues
Specific Activity	> 100,000 units/mg protein
Purity (SDS-PAGE)	> 95%
DNase Activity	Not detectable
Protease Activity	Not detectable
Non-specific RNase Activity	Not detectable
pH (Storage Buffer)	7.5 ± 0.5

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.