Description

Ec 3.1.3.25 CAS NO 37184-63-7 is a high-purity enzyme preparation, specifically a 3'-nucleotidase, which plays a critical role in nucleotide metabolism. This product is essential for research and industrial applications requiring precise enzymatic activity to hydrolyze 3'-nucleotides. It is primarily utilized by biotechnology firms, pharmaceutical research laboratories, and diagnostic kit manufacturers for biochemical assays, substrate specificity studies, and as a reagent in nucleotide sequencing workflows.

Application

• Biochemical Research & Assay Development: Used as a key reagent in enzymatic studies, metabolic pathway analysis, and the development of colorimetric or fluorometric assays.
• Pharmaceutical R&D: Employed in target validation, screening for enzyme inhibitors, and studying nucleotide-related drug mechanisms.
• Diagnostic Manufacturing: Incorporated as a component in diagnostic kits for clinical chemistry, particularly those measuring nucleotide levels.
• Enzyme Engineering & Characterization: Serves as a standard or substrate for characterizing enzyme kinetics, stability, and mutant variants.
• Nucleic Acid Analysis: Used in sequencing techniques and protocols that require the specific removal of 3'-phosphate groups from nucleotides or oligonucleotides.
• Academic Research: Fundamental tool in university and institutional labs for molecular biology and biochemistry education and discovery.

Basic Information

Product Name	Ec 3.1.3.25
CAS No.	37184-63-7
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	3'-Nucleotidase; EC 3.1.3.25; 3'-Ribonucleotidase; Uridine 3'-nucleotidase; 3'-Mononucleotidase; Nucleotide 3'-phosphatase; 3'-Phosphatase
EINECS	Contact for details

Quality Control

Our Ec 3.1.3.25 is produced under stringent quality management systems. Each batch is tested to ensure high specific activity, purity, and consistency. We provide comprehensive Certificates of Analysis (COA) detailing parameters such as activity units, protein content, and impurity profiles. Our quality commitment aligns with standards suitable for research and industrial use, ensuring reliable performance in your applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This material is hygroscopic (moisture-sensitive); ensure containers are sealed tightly after use to prevent moisture absorption. For short-term use, aliquoting is recommended to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or solution
Identification (Activity)	Conforms
Specific Activity	≥ [Value] units/mg protein
Purity (SDS-PAGE)	≥ 90%
Protein Content (Bradford)	Reported on COA
pH (in solution)	7.0 - 7.5
Endotoxin (LAL)	< 1.0 EU/μg (if required)
Biological Activity	Confirmed via standard assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.