Description

4-Methylumbelliferyl-n-Acetyl-β-D-Galactosaminide Hydrate is a specialized fluorogenic substrate used for the detection and quantification of N-acetyl-β-D-galactosaminidase (NAGase) activity. This compound matters because it provides a highly sensitive and specific means for enzymatic assays, crucial for research and diagnostic applications. It is primarily needed by professionals in the life sciences, pharmaceutical R&D, and clinical diagnostics sectors for studying lysosomal storage disorders, biomarker research, and high-throughput screening.

Application

• Enzymatic Assay Substrate: Primary fluorogenic substrate for the detection and kinetic study of N-acetyl-β-D-galactosaminidase (NAGase) activity.
• Biomedical Research: Used in research related to lysosomal storage disorders, such as Tay-Sachs and Sandhoff diseases, where NAGase activity is a key biomarker.
• Diagnostic Reagent Manufacturing: A critical raw material in the formulation of diagnostic kits for measuring NAGase levels in biological samples (e.g., urine, serum).
• High-Throughput Screening (HTS): Employed in pharmaceutical discovery for screening potential enzyme inhibitors or activators in microplate-based assays.
• Cell Biology & Biochemistry: Utilized for in vitro and in situ enzymatic activity staining in cellular and tissue studies.
• Quality Control in Bioprocessing: Applied to monitor enzyme activity during the purification and production of recombinant proteins.

Basic Information

Product Name	4-Methylumbelliferyl-n-Acetyl-β-D-Galactosaminide Hydrate
CAS No.	36476-29-6
Molecular Formula	C18H22NO10 · xH2O
Molecular Weight	396.37 g/mol (Anhydrous basis)
Synonyms	4-MU-β-GalNAc; 4-Methylumbelliferyl N-acetyl-β-D-galactosaminide; 4-Methylumbelliferyl 2-acetamido-2-deoxy-β-D-galactopyranoside; NAGase Substrate; 4-MU-GalNAc; 4-Methylumbelliferyl β-D-N-acetylgalactosaminide; Fluorogenic NAG substrate
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferyl-n-Acetyl-β-D-Galactosaminide Hydrate is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) with detailed specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. The product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 5.0%
Fluorogenic Activity	Conforms (Enzymatic Test)
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.