Description

Tetracycline, [7-3H(N)] is a high-specific-activity, tritium-labeled derivative of the broad-spectrum tetracycline antibiotic. This radiolabeled compound is essential for advanced research requiring precise tracking and quantification in biological systems. It is a critical tool for scientists in pharmaceutical development, metabolic studies, and antimicrobial research who require reliable tracers for autoradiography, receptor binding assays, and ADME (Absorption, Distribution, Metabolism, and Excretion) investigations.

Application

• Pharmacokinetic and Metabolic Studies: Used as a tracer to study the absorption, distribution, metabolism, and excretion (ADME) of tetracycline and related compounds in vivo and in vitro.
• Mechanism of Action Research: Employed in receptor binding assays and autoradiography to investigate the specific interactions of tetracycline with bacterial ribosomes and cellular targets.
• Antimicrobial Resistance Research: Facilitates studies on drug uptake, efflux mechanisms, and the binding affinity of tetracycline in resistant bacterial strains.
• Biochemical Pathway Analysis: Tracks the incorporation and fate of tetracycline in complex biological systems and environmental samples.
• Drug Development Screening: Serves as a key reagent in high-throughput screening assays for discovering new tetracycline-based antibiotics or potentiators.
• Quality Control & Reference Standard: Can be utilized as an internal standard in advanced analytical methods like LC-MS for quantifying tetracycline levels.

Basic Information

Product Name	Tetracycline, [7-3H(N)]
CAS No.	36051-40-8
Molecular Formula	C₂₂H₂₄N₂O₈ • xH₂O (Tritiated)
Molecular Weight	~444.4 g/mol (unlabeled base)
Synonyms	[7-³H]Tetracycline; Tritiated Tetracycline; Tetracycline (tritiated); 4-(Dimethylamino)-1,4,4a,5,5a,6,11,12a-octahydro-3,6,10,12,12a-pentahydroxy-6-methyl-1,11-dioxo-2-naphthacenecarboxamide (³H-labeled); Tetracycline-7-³H; NSC-39511 (labeled form); Antibiotic TC (labeled)
EINECS	Contact for details

Quality Control

Every batch of Tetracycline, [7-3H(N)] is manufactured and analyzed under controlled conditions to ensure high radiochemical purity and specific activity. Quality assurance includes rigorous testing via HPLC with radiometric detection. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing specific activity (Ci/mmol or GBq/mmol), radiochemical purity, chemical purity, and solvent specifications. Our production adheres to GLP (Good Laboratory Practice) standards to guarantee consistency and reliability for critical research applications.

Storage

Preserve in a tightly closed container, protected from light. Store at or below -20°C. This product is hygroscopic (moisture-sensitive). For long-term storage, keep the primary container in a sealed secondary container with desiccant. Always handle in accordance with all applicable radiation safety regulations and guidelines.

Specification

Item	Specification
Appearance	Yellow to Orange Solid or Solution
Specific Activity	10-30 Ci/mmol (370-1110 GBq/mmol) [Custom ranges available]
Radiochemical Purity	≥ 95% (by HPLC)
Chemical Purity	≥ 90% (by HPLC, UV detection)
Identification	HPLC co-elution with reference standard
Solvent	Ethanol/Water or custom solvent upon request
Concentration	1.0 mCi/mL (37 MBq/mL) or as specified

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.