Description

3-Indoxyl-β-D-Glucuronic Acid Cyclohexylammonium Salt is a high-purity biochemical reagent primarily used as a chromogenic substrate for the detection of β-glucuronidase enzyme activity. This compound is critical for researchers and quality control laboratories requiring precise and sensitive enzymatic assays. It is essential for applications in molecular biology, microbiology, and diagnostic kit manufacturing, where reliable substrate performance is non-negotiable.

Application

• Enzymatic Substrate for β-Glucuronidase (GUS) Assays: Widely used in molecular biology and genetic engineering as a key reagent in GUS reporter gene systems to monitor gene expression.
• Microbiological Identification: Employed in selective media and diagnostic tests, such as those for E. coli, to differentiate bacterial strains based on β-glucuronidase activity.
• Diagnostic Kit Manufacturing: Serves as a core component in the production of clinical and veterinary diagnostic kits for enzymatic detection.
• Plant Biotechnology Research: Used in plant science to study promoter activity and transformation efficiency via the GUS assay.
• Food Safety Testing: Applied in methods for the rapid detection of fecal coliforms and specific pathogens in food and water samples.
• Pharmaceutical Research: Utilized in drug discovery and development for screening compounds that modulate enzyme activity.

Basic Information

Product Name	3-Indoxyl-β-D-Glucuronic Acid Cyclohexylammonium Salt
CAS No.	35804-66-1
Molecular Formula	C20H32N2O7
Molecular Weight	412.48 g/mol
Synonyms	5-Bromo-4-chloro-3-indolyl-β-D-glucuronic acid cyclohexylammonium salt; X-Glucuronide, Cyclohexylammonium salt; 5-Bromo-4-chloro-3-indolyl β-D-glucopyranosiduronic acid cyclohexylamine salt; BCIG; X-GlucA; GUS Substrate; 3-Indolyl-β-D-glucuronic acid cyclohexylammonium salt; Indoxyl Glucuronide Cyclohexylammonium Salt
EINECS	Contact for details

Quality Control

Our 3-Indoxyl-β-D-Glucuronic Acid Cyclohexylammonium Salt is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive biochemical applications. Certificates of Analysis (COA) with detailed specifications are provided to guarantee batch-to-batch consistency and traceability.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	Off-white to light yellow powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Specific Rotation	Contact for details
Heavy Metals	< 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.