Description

4-Nitrophenyl-β-D-Mannopyranoside is a specialized chromogenic substrate used in enzymatic assays. Its core value lies in its ability to act as a specific substrate for mannosidase enzymes, producing a quantifiable colorimetric signal upon cleavage. This compound is essential for researchers and manufacturers in the life sciences, pharmaceutical development, and diagnostic industries who require precise tools for enzyme activity measurement and inhibition studies.

Application

• Enzymatic Research: Primary substrate for the detection and kinetic analysis of α-mannosidase and other glycosidase enzymes.
• Diagnostic Assay Development: Used in the formulation of clinical diagnostic kits for the detection of specific enzyme deficiencies or activities.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify potential glycosidase inhibitors for drug discovery.
• Biochemical Reagent: A key component in research-grade reagent mixtures for studying carbohydrate metabolism and processing.
• Quality Control in Bioprocessing: Monitors enzyme activity and consistency in the production of therapeutic proteins and other biologics.

Basic Information

Product Name	4-Nitrophenyl-β-D-Mannopyranoside
CAS No.	35599-02-1
Molecular Formula	C12H15NO8
Molecular Weight	301.25 g/mol
Synonyms	p-Nitrophenyl β-D-Mannopyranoside; 4-Nitrophenyl β-D-Mannoside; β-4-Nitrophenyl mannopyranoside; PNPM; p-Nitrophenyl mannoside; 4-NP-β-D-mannopyranoside; (2R,3S,4S,5S,6R)-2-(Hydroxymethyl)-6-(4-nitrophenoxy)oxane-3,4,5-triol
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl-β-D-Mannopyranoside is manufactured under a strict quality management system. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification and identity confirmation by IR and NMR spectroscopy, to ensure it meets the high standards required for research and diagnostic applications. A detailed Certificate of Analysis (COA) is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Water Content (KF)	≤ 2.0%
Specific Rotation	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.