Description

D-1,4-Dithiothreitol (DTT) is a high-purity, small-molecule reducing agent essential for cleaving and preventing the reformation of disulfide bonds in proteins and peptides. Its strong reducing power and specificity make it a critical reagent for maintaining protein structure and function in sensitive biochemical processes. This product is indispensable for researchers and manufacturers in the biotechnology, pharmaceutical, and diagnostic industries who require reliable and consistent performance in applications such as protein purification, electrophoresis, and molecular biology.

Application

• Protein Biochemistry & Purification: Used to reduce and denature proteins for SDS-PAGE analysis, chromatography, and structural studies.
• Pharmaceutical Development: Acts as a stabilizing agent and reducing component in formulation buffers for therapeutic proteins and antibodies.
• Molecular Biology: Essential for maintaining the activity of sulfhydryl-dependent enzymes (e.g., reverse transcriptase, DNA polymerase) and in nucleic acid hybridization buffers.
• Diagnostic Reagent Manufacturing: Incorporated into assay kits, such as ELISA and lateral flow tests, to ensure proper antibody-antigen binding.
• Cell Culture & Biotechnology: Used in media and lysis buffers to create a reducing environment for cell growth and protein extraction.
• Chemical Synthesis: Serves as a selective reducing agent in organic synthesis and as a protecting group for thiols.

Basic Information

Product Name	D-1,4-Dithiothreitol
CAS No.	35454-97-8
Molecular Formula	C4H10O2S2
Molecular Weight	154.25 g/mol
Synonyms	DTT; Cleland's Reagent; (2R,3R)-1,4-Dimercaptobutane-2,3-diol; Dithiothreitol; D-1,4-Dithio-L-threitol; D-Threo-1,4-dimercapto-2,3-butanediol; (R,R)-Dithiothreitol; Erythro-1,4-dimercapto-2,3-butanediol
EINECS	Contact for details

Quality Control

Our D-1,4-Dithiothreitol is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity. Each lot is subjected to comprehensive analytical testing, including HPLC for assay and purity, and meets stringent specifications for critical parameters such as optical rotation and residue on ignition. Certificates of Analysis (COA) documenting all test results are provided and available upon request to support your quality assurance and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C under an inert atmosphere (e.g., nitrogen or argon) to minimize oxidation and moisture absorption. For long-term stability, keep the product in a cool, dry, and well-ventilated area away from strong oxidizing agents.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference standard
Assay (HPLC)	≥ 98.0%
Optical Rotation [α]20/D	-19.0° to -22.0° (c=1 in water)
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Heavy Metals (as Pb)	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.