Description

Adenosine-5'-o-(1-Thiotriphosphate), Sp-Isomer Sodium Salt CAS NO 35094-46-3 is a high-purity, non-hydrolyzable ATP analog where one of the non-bridging oxygen atoms in the γ-phosphate group is replaced by sulfur, specifically in the Sp configuration. This compound is critical for studying ATP-dependent enzymes and signaling pathways, as it acts as a potent inhibitor or a substrate analog resistant to hydrolysis. It is an essential biochemical reagent for researchers and manufacturers in molecular biology, enzymology, and pharmaceutical development, particularly for kinase and ATPase assays.

Application

• Enzyme Kinetics & Mechanism Studies: Used as a substrate analog to investigate the stereochemical course and mechanism of ATP-dependent enzymes like kinases, ATPases, and polymerases.
• Biochemical Assay Development: Serves as a critical component in high-throughput screening (HTS) assays for drug discovery targeting ATP-binding sites.
• Signal Transduction Research: Employed in studying phosphorylation events and nucleotide-dependent signaling cascades within cells.
• Structural Biology: Used in X-ray crystallography and NMR studies to obtain enzyme-ligand complex structures, providing insights into active site geometry.
• Diagnostic Reagent Manufacturing: A key raw material in producing diagnostic kits that measure kinase activity or ATP concentration.
• Pharmaceutical R&D: Utilized in the development and testing of novel therapeutic agents that modulate ATP-utilizing enzymes.

Basic Information

Product Name	Adenosine-5'-o-(1-Thiotriphosphate), Sp-Isomer Sodium Salt
CAS No.	35094-46-3
Molecular Formula	C10H14N5O12P3S · xNa+
Molecular Weight	Approx. 551.1 (Free acid) + Na+. Contact for details on specific salt form.
Synonyms	ATPγS (Sp-isomer); Adenosine 5'-(γ-thiotriphosphate), Sp diastereomer; Sp-ATP-γ-S sodium salt; Adenosine-5'-(3-thiotriphosphate) Sp-isomer; 5'-Adenylyl (γ-thio)triphosphate, Sp isomer; γ-Thio-ATP (Sp); 1-Thioadenosine-5'-triphosphate Sp-isomer sodium salt
EINECS	Contact for details.

Quality Control

Our Adenosine-5'-o-(1-Thiotriphosphate), Sp-Isomer Sodium Salt is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing to ensure high purity, correct stereochemistry, and batch-to-batch consistency. Certificates of Analysis (COA) are provided, detailing parameters such as HPLC purity, diastereomeric excess, NMR identification, and endotoxin levels where applicable. We support compliance with cGMP and ISO 9001 standards for research and diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccant and under an inert atmosphere. Allow the product to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Diastereomeric Purity (Sp-isomer)	≥ 98%
Water Content (KF)	≤ 5.0%
pH (in solution)	6.0 - 8.0
Biological Activity (Enzymatic Assay)	Conforms

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.