Description

Adenosine 5'-o-(2-Thiodiphosphate) is a chemically modified nucleotide analog where a sulfur atom replaces one of the non-bridging oxygen atoms in the beta phosphate group of ADP. This structural modification makes it a valuable, hydrolysis-resistant tool for probing the mechanisms of ATP-dependent enzymes and nucleotide-binding proteins. It is primarily used by researchers in biochemistry, enzymology, and molecular pharmacology to study kinase activity, nucleotide signaling, and energy metabolism pathways. This high-purity biochemical is essential for advanced life science research and diagnostic assay development.

Application

• Enzymology Research: Used as a substrate analog to study the kinetics and inhibition of ATPases, kinases, and other phosphotransferase enzymes.
• Signal Transduction Studies: Employed in investigations of purinergic receptor signaling and nucleotide-mediated cellular communication.
• Structural Biology: Aids in X-ray crystallography and NMR studies to determine the structure of nucleotide-binding protein pockets.
• Diagnostic Assay Development: Serves as a key component in developing high-sensitivity assays for enzyme activity and drug screening.
• Metabolic Pathway Analysis: Utilized as a tracer or inhibitor to dissect complex energy metabolism and biosynthesis pathways.
• Pharmaceutical Research: Acts as a lead compound or tool for identifying and validating new therapeutic targets in areas like oncology and neurology.

Basic Information

Product Name	Adenosine 5'-o-(2-Thiodiphosphate)
CAS No.	35094-45-2
Molecular Formula	C10H15N5O9P2S
Molecular Weight	459.26 g/mol
Synonyms	Adenosine 5'-(2-Thiodiphosphate); Adenosine-5'-O-[2-thiodiphosphate]; ADP-β-S; ADPβS; Adenosine 5'-[β-thio]diphosphate; 2'-Deoxyadenosine-5'-O-[2-thiodiphosphate] (common misnomer, verify structure); ADENOSINE-5'-DIPHOSPHOROTHIOATE; ATP analog (β-thio)
EINECS	Contact for details

Quality Control

Our Adenosine 5'-o-(2-Thiodiphosphate) is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity assessment, NMR for structural confirmation, and mass spectrometry. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with research-grade specifications. Our quality commitment supports reproducible and reliable results for your critical research applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder or lyophilized solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 3.0%
Solubility	Soluble in water or buffer solutions

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.