Description

2-Chloro-4-Nitrophenyl-2,3,4,6-Tetra-o-Acetyl-β-D-Glucopyranoside is a high-purity, synthetically derived glycoside derivative that serves as a critical chromogenic substrate for enzymatic assays. Its core value lies in providing a reliable and sensitive means to detect and quantify the activity of specific glycosidases, such as β-glucosidase, in research and diagnostic applications. This compound is essential for researchers and manufacturers in the life sciences, pharmaceutical development, and clinical diagnostics sectors who require precise biochemical tools for enzyme kinetics and diagnostic kit formulation.

Application

• Enzymatic Substrate for β-Glucosidase: Primary use as a chromogenic substrate in biochemical assays to measure β-glucosidase activity through the release of a colored product.
• Diagnostic Reagent Manufacturing: A key raw material in the production of diagnostic kits and reagents for clinical and research laboratories.
• Biochemical Research: Used in enzyme kinetics studies, inhibitor screening, and characterization of glycosidase enzymes in academic and industrial research settings.
• Pharmaceutical Development: Employed in drug discovery programs targeting glycosidase enzymes, particularly for high-throughput screening (HTS) assays.
• Quality Control in Biotechnology: Acts as a standard or reference compound for validating enzyme purity and activity in biomanufacturing processes.
• Substrate for Other Glycosidases: May be utilized, with appropriate validation, as a substrate for related hydrolytic enzymes in specialized assay systems.

Basic Information

Product Name	2-Chloro-4-Nitrophenyl-2,3,4,6-Tetra-o-Acetyl-β-D-Glucopyranoside
CAS No.	35023-71-3
Molecular Formula	C22H24ClNO12
Molecular Weight	529.88 g/mol
Synonyms	2-Chloro-4-nitrophenyl β-D-glucopyranoside tetraacetate; 4-Nitrophenyl 2-chloro-4-nitrophenyl β-D-glucopyranoside tetraacetate; p-Nitrophenyl 2-chloro-4-nitrophenyl β-D-glucopyranoside tetraacetate; 2-Chloro-4-nitrophenyl β-D-glucoside tetraacetate; CNP-Glc tetraacetate; β-Glucosidase substrate (chromogenic, acetylated); 2-Chloro-4-nitrophenyl 2,3,4,6-tetra-O-acetyl-β-D-glucopyranoside
EINECS	Contact for details

Quality Control

Our 2-Chloro-4-Nitrophenyl-2,3,4,6-Tetra-o-Acetyl-β-D-Glucopyranoside is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity suitable for sensitive biochemical applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods. Certificates of Analysis (COA) detailing all specifications and test results are provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. This compound is light-sensitive (store away from light) and may be hygroscopic (moisture-sensitive); therefore, containers should be kept sealed and desiccated when not in use to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Specific Rotation	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.