Description

Biotin-Nhs CAS NO 35013-72-0 is a high-purity, activated ester derivative of biotin, specifically designed for efficient bioconjugation. This compound is critical for labeling biomolecules, enabling their detection, isolation, and analysis in advanced research and diagnostics. It is an essential reagent for scientists and manufacturers in the pharmaceutical, biotechnology, and life science research sectors who require reliable and specific protein or nucleic acid tagging.

Application

• Bioconjugation & Protein Labeling: Primary reagent for covalently attaching biotin to antibodies, enzymes, and other proteins for use in immunoassays (ELISA, Western Blot) and pull-down assays.
• Affinity Chromatography Probe Development: Used to create biotinylated ligands for immobilization on streptavidin/avidin resins to purify target molecules.
• Diagnostic Kit Manufacturing: A key component in the production of diagnostic test strips and kits that utilize biotin-streptavidin technology for signal amplification.
• Cell Surface Labeling: Facilitates the tagging of cell surface proteins or receptors for flow cytometry (FACS) and microscopy studies.
• Nucleic Acid Probes: Employed in the synthesis of biotin-labeled DNA or RNA probes for in situ hybridization and microarray applications.
• Drug Delivery & Targeting Research: Used in preclinical research to conjugate targeting moieties to drug carriers or therapeutic payloads.

Basic Information

Product Name	Biotin-Nhs
CAS No.	35013-72-0
Molecular Formula	C14H19N3O4S
Molecular Weight	325.38 g/mol
Synonyms	Biotin N-hydroxysuccinimide ester; (+)-Biotin N-succinimidyl ester; D-Biotin N-succinimidyl ester; Biotin succinimidyl ester; Biotin-SE; Biotin-OSu; N-Biotinoyloxysuccinimide; 5-[(3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]-N-hydroxypentanamide N-succinimidyl ester
EINECS	Contact for details

Quality Control

Our Biotin-Nhs is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research and diagnostic applications. Certificates of Analysis (COA) with detailed specifications are available upon request to support your quality assurance and regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.