Description

Dl-Dithiothreitol CAS NO 34834-12-3 is a versatile and essential reducing agent, widely recognized for its ability to cleave disulfide bonds in proteins and peptides. Its primary value lies in stabilizing sulfhydryl groups and preventing unwanted oxidation in sensitive biochemical systems. This compound is a critical reagent for researchers and manufacturers in the biotechnology, pharmaceutical, and diagnostic industries, where precise molecular manipulation is required.

Application

• Protein Biochemistry: Reduction and denaturation of proteins for electrophoresis (e.g., SDS-PAGE) and structural analysis.
• Pharmaceutical Research: Used in the synthesis and purification of biopharmaceuticals, including monoclonal antibodies and recombinant proteins.
• Molecular Biology: Protection of thiol groups in enzymatic reactions and nucleic acid manipulation to maintain activity.
• Diagnostic Kit Manufacturing: A key component in stabilizing reagents for immunoassays and clinical test kits.
• Cell Culture & Media Preparation: Acts as an antioxidant in specialized cell culture media to promote cell growth and viability.
• Chemical Synthesis: Serves as a selective reducing agent in organic synthesis for the production of fine chemicals and intermediates.

Basic Information

Item	Detail
Product Name	Dl-Dithiothreitol
CAS No.	3483-12-3
Molecular Formula	C4H10O2S2
Molecular Weight	154.25 g/mol
Synonyms	DTT; DL-Dithiothreitol; (±)-1,4-Dithiothreitol; *three*-1,4-Dimercapto-2,3-butanediol; Cleland's Reagent; 1,4-Dithio-*DL*-threitol; Dithioerythritol (isomer); DTE; 2,3-Dihydroxybutane-1,4-dithiol
EINECS	Contact for details

Quality Control

Our Dl-Dithiothreitol is produced under a stringent quality management system. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and industrial applications. Certificates of Analysis (COA) documenting purity, appearance, and specific tests are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Due to its hygroscopic and easily oxidized nature, store under an inert atmosphere (e.g., nitrogen or argon) at a controlled room temperature (15-25°C). For long-term stability, desiccants are recommended within the storage container.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Melting Point	40 - 44 °C
Water Content (KF)	≤ 2.0%
Heavy Metals (as Pb)	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.