Description

L-Luciferin is the natural substrate for the firefly luciferase enzyme, essential for generating bioluminescent light. This high-purity compound is critical for sensitive, quantitative assays in life science research and drug discovery. It is primarily utilized by researchers and quality control professionals in the pharmaceutical, biotechnology, and academic sectors for applications including reporter gene assays, ATP detection, and high-throughput screening.

Application

• Reporter Gene Assays: As the key substrate in firefly luciferase-based systems for monitoring gene expression and promoter activity.
• ATP Detection & Quantification: For highly sensitive measurement of adenosine triphosphate (ATP) in cell viability, cytotoxicity, and microbial contamination tests.
• High-Throughput Screening (HTS): Enabling luminescent readouts in drug discovery campaigns for targets such as kinases, GPCRs, and proteases.
• Bioluminescence Imaging (BLI): Used in vivo for non-invasive tracking of tumor growth, metastasis, and treatment efficacy in preclinical animal models.
• Cell-Based Assays: For real-time monitoring of intracellular processes including apoptosis, signal transduction, and metabolic activity.
• Microbial Detection: Rapid, sensitive testing for bacterial contamination in sterile products and environmental samples.

Basic Information

Product Name	L-Luciferin
CAS No.	34500-31-7
Molecular Formula	C11H8N2O3S2
Molecular Weight	280.32 g/mol
Synonyms	D-(-)-Luciferin; (-)-Luciferin; Firefly Luciferin; (4S)-4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid; Beetle Luciferin; LH2; D-Luciferin (incorrect but common alias); Luciferin, firefly
EINECS	Contact for details

Quality Control

Our L-Luciferin is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in a standard luciferase assay. We ensure compliance with relevant quality benchmarks for research-grade biochemicals.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to minimize oxidation. For long-term storage, desiccate to protect against moisture. Allow the product to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to reference spectrum
Assay (HPLC)	≥98.0%
Bioluminescent Activity	≥90% of reference standard
Water Content (KF)	≤1.0%
Residue on Ignition	≤0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.