Description

4-Methylumbelliferyl-p-Guanidinobenzoate Hydrochloride is a specialized fluorogenic substrate used for the sensitive detection and quantification of serine proteases. This compound matters for its high specificity and sensitivity in enzymatic assays, enabling critical research and diagnostic applications. It is primarily needed by researchers and manufacturers in the life sciences, pharmaceutical development, and clinical diagnostics sectors for studying enzyme kinetics and developing diagnostic kits.

Application

• Protease Activity Assays: A key fluorogenic substrate for detecting and quantifying serine protease activity, such as trypsin, thrombin, and plasmin.
• Biochemical Research: Used in enzyme kinetics studies to determine kinetic parameters (Km, Vmax) and inhibition constants (Ki).
• Diagnostic Kit Development: Employed in the formulation of diagnostic kits for conditions related to protease imbalances, including coagulation disorders and inflammatory diseases.
• Pharmaceutical Screening: Utilized in high-throughput screening (HTS) platforms to identify and characterize novel serine protease inhibitors as potential drug candidates.
• Cell Biology Studies: Applied in assays to monitor protease activity within cellular systems and tissue samples.
• Quality Control in Biologics: Serves as a reagent for testing the activity and purity of therapeutic enzymes during biomanufacturing processes.

Basic Information

Product Name	4-Methylumbelliferyl-p-Guanidinobenzoate Hydrochloride
CAS No.	34197-46-1
Molecular Formula	C18H16N4O5 • HCl
Molecular Weight	404.80 g/mol (Free base: 368.34 g/mol)
Synonyms	4-MUGB • 4-Methylumbelliferyl 4-guanidinobenzoate hydrochloride • MUG-p-Guanidinobenzoate HCl • p-Guanidinobenzoic acid 4-methylumbelliferyl ester hydrochloride • 4-Methylumbelliferyl p-guanidinobenzoate HCl • 7-Hydroxy-4-methylcoumarin 4-guanidinobenzoate hydrochloride • Fluorogenic trypsin substrate • Protease substrate MUGB
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferyl-p-Guanidinobenzoate Hydrochloride is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research and diagnostic applications. Certificates of Analysis (COA) with detailed specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Fluorogenic Activity	Functional in standard trypsin assay
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.