Description

2-Naphthyl-β-D-Galactopyranoside is a specialized chromogenic and fluorogenic substrate used extensively in enzymatic assays. This compound matters for its high specificity in detecting β-galactosidase activity, a critical enzyme marker in molecular biology and diagnostic applications. It is primarily needed by research institutions, diagnostic kit manufacturers, and pharmaceutical companies for applications in gene expression studies, reporter assays, and microbiological testing.

Application

• Enzymatic Substrate for β-Galactosidase: Used as a chromogenic substrate in histochemical staining and microbiological media to detect enzyme activity.
• Molecular Biology Research: Employed in reporter gene assays (e.g., lacZ gene) to study gene regulation and promoter activity.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits for detecting specific bacterial enzymes (e.g., in E. coli).
• Biochemical Research: Utilized in enzyme kinetics studies to measure β-galactosidase kinetics and inhibition.
• Cell & Tissue Staining: Applied in immunohistochemistry and in situ hybridization techniques for visualizing gene expression patterns.
• Pharmaceutical Screening: Used in high-throughput screening (HTS) assays to identify modulators of β-galactosidase activity for drug discovery.

Basic Information

Product Name	2-Naphthyl-β-D-Galactopyranoside
CAS No.	33993-25-8
Molecular Formula	C16H18O6
Molecular Weight	306.31 g/mol
Synonyms	2-Naphthyl β-D-galactopyranoside; 6-β-Galactopyranosyl-2-naphthol; β-D-Galactopyranoside, 2-naphthyl; 2-Naphthol, 6-β-D-galactopyranosyl-; 2-Naphthyl-β-D-galactoside; 2-Naphthyl β-D-galactoside; ONPG Analog (for β-galactosidase); 2-Naphthylgalactoside
EINECS	Contact for details

Quality Control

Our 2-Naphthyl-β-D-Galactopyranoside is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation by IR and NMR spectroscopy, to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and light-sensitive; therefore, containers must be kept tightly sealed and in the original packaging until use to prevent degradation and absorption of moisture.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Melting Point	Contact for details
Specific Rotation	Contact for details
Heavy Metals	< 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.