Description

6-(2,2,6,6-Tetramethylpiperidine-1-Oxyl)-Adenosine Triphosphate is a spin-labeled derivative of adenosine triphosphate (ATP), a critical molecule in cellular energy transfer. This compound is specifically engineered for advanced research applications, particularly in electron paramagnetic resonance (EPR) spectroscopy, where it serves as a powerful probe for studying enzyme kinetics, conformational changes, and binding interactions. It is an essential tool for biochemists, molecular biologists, and pharmaceutical researchers working on nucleotide-dependent processes, ATPase mechanisms, and structural biology.

Application

• EPR/ESR Spectroscopy: As a paramagnetic spin label to investigate the structure, dynamics, and binding sites of ATP-binding proteins and enzymes.
• Enzyme Mechanism Studies: Probing the active sites and catalytic cycles of kinases, ATPases, synthases, and other ATP-dependent enzymes.
• Membrane Protein Research: Studying conformational changes in membrane-bound transporters and channels that utilize ATP.
• Structural Biology: Aiding in the determination of protein-ligand complex structures and dynamics through magnetic resonance techniques.
• Drug Discovery Screening: Serving as a tool in high-throughput assays to identify inhibitors or modulators of ATP-binding targets.
• Biophysical Probes: Investigating real-time nucleotide exchange and hydrolysis events in solution.

Basic Information

Product Name	6-(2,2,6,6-Tetramethylpiperidine-1-Oxyl)-Adenosine Triphosphate
CAS No.	33913-54-1
Molecular Formula	C20H33N7O14P3
Molecular Weight	688.43 g/mol
Synonyms	6-TEMPO-ATP; TEMPO-ATP; ATP-TEMPO; Adenosine-5'-triphosphate, 6-(2,2,6,6-tetramethyl-1-oxyl-4-piperidinyl) deriv.; Spin-labeled ATP; SL-ATP; 6-(2,2,6,6-Tetramethylpiperidin-1-yloxyl)adenosine triphosphate; 6-(2,2,6,6-Tetramethyl-1-piperidinyloxy)adenosine 5'-triphosphate
EINECS	Contact for details

Quality Control

Our 6-(2,2,6,6-Tetramethylpiperidine-1-Oxyl)-Adenosine Triphosphate is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot is analyzed via HPLC, NMR, and EPR spectroscopy to confirm identity, purity, and spin label integrity. Certificates of Analysis (COA) detailing all specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, consider desiccant and under an inert atmosphere to maintain stability.

Specification

Item	Specification
Appearance	Yellow to orange powder or solid
Identification (HPLC)	Conforms
Identification (EPR)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 3.0%
Specific Activity (EPR)	Contact for details
Biological Activity	Tested in standard ATPase assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.