Description

D-Biotin p-Nitrophenyl Ester is a high-purity, activated ester derivative of the essential vitamin biotin. This compound is a critical reagent for the bioconjugation and labeling of biomolecules, enabling the study of protein interactions and cellular processes. It is primarily utilized by researchers and manufacturers in the fields of life sciences, diagnostics, and pharmaceutical development for creating biotinylated probes and affinity-based assays.

Application

• Bioconjugation Reagent: Key intermediate for covalently attaching biotin tags to proteins, antibodies, peptides, and nucleic acids.
• Affinity Chromatography Ligand Synthesis: Used to functionalize solid supports (e.g., agarose beads) for streptavidin/avidin-based purification systems.
• Diagnostic Assay Development: Essential for labeling detection antibodies or antigens in ELISA, lateral flow assays, and other immunoassays.
• Chemical Biology & Proteomics: Employed in activity-based protein profiling (ABPP) and the study of protein-protein interactions.
• Drug Delivery Systems: Facilitates the targeted delivery of therapeutic agents through biotin-streptavidin technology.
• Cell Surface Labeling: Used to tag membrane proteins for visualization and tracking in fluorescence microscopy and flow cytometry.

Basic Information

Product Name	D-Biotin p-Nitrophenyl Ester
CAS No.	33755-53-2
Molecular Formula	C16H19N3O5S
Molecular Weight	365.41 g/mol
Synonyms	Biotin 4-nitrophenyl ester; (+)-Biotin p-nitrophenyl ester; D-(+)-Biotin p-nitrophenyl ester; Biotin pNPE; Biotin-PNP; Vitamin H p-nitrophenyl ester; 5-[(3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoic acid 4-nitrophenyl ester
EINECS	Contact for details

Quality Control

Our D-Biotin p-Nitrophenyl Ester is manufactured under strict quality systems to ensure batch-to-batch consistency and high chemical purity. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing key parameters such as HPLC purity, identity confirmation (IR, NMR), and moisture content. We support research and production needs with material traceability and documentation in compliance with cGMP principles where applicable.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as indicated on the label or COA. This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and reactivity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO; slightly soluble in organic solvents

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.