Description

Diammonium 4-Nitrophenyl Phosphate is a high-purity chromogenic substrate essential for enzymatic assays. This compound serves as a critical reagent for detecting and quantifying the activity of alkaline phosphatase and other phosphatases in diagnostic and research applications. It is primarily needed by manufacturers of in-vitro diagnostic kits, biochemical research laboratories, and quality control departments in the pharmaceutical and biotechnology industries.

Application

• Primary Substrate for Alkaline Phosphatase (ALP) Assays: Used as the key component in clinical chemistry reagents for measuring ALP activity in serum, a vital diagnostic marker for liver and bone disorders.
• Enzyme-Linked Immunosorbent Assay (ELISA): Employed in colorimetric ELISA protocols where alkaline phosphatase is the reporter enzyme, generating a measurable yellow-colored product (p-nitrophenol).
• Molecular Biology Research: Utilized in nucleic acid detection systems (e.g., Southern, Northern, and Western blots) that use alkaline phosphatase-conjugated probes.
• Pharmaceutical Quality Control: Applied in potency testing and enzymatic activity assays for drug products and raw materials during manufacturing.
• Food Safety Testing: Incorporated into rapid test kits for detecting bacterial contamination, as certain bacteria produce alkaline phosphatase.
• Biochemical Reagent Manufacturing: Serves as a raw material for formulating standardized diagnostic kits and research-grade biochemicals.

Basic Information

Product Name	Diammonium 4-Nitrophenyl Phosphate
CAS No.	32348-91-7
Molecular Formula	C6H11N2O6P
Molecular Weight	238.14 g/mol
Synonyms	4-Nitrophenyl Phosphate Diammonium Salt; p-Nitrophenyl Phosphate Diammonium Salt; PNPP Diammonium Salt; Phosphoric Acid 4-Nitrophenyl Ester Diammonium Salt; Alkaline Phosphatase Substrate; ALP Substrate; Di-Ammonium 4-Nitrophenyl Phosphate; 4-NPP Diammonium Salt
EINECS	Contact for details

Quality Control

Our Diammonium 4-Nitrophenyl Phosphate is manufactured under a strict quality management system. Each batch is tested to ensure high purity, low background absorbance, and optimal performance in enzymatic assays. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing specifications such as assay (by HPLC), absorbance profile, solubility, and limits for impurities. We support compliance with ISO 13485 standards for in-vitro diagnostic manufacturers.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture. For long-term stability, consider storage under desiccant.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Absorbance (1% Solution)	Meets internal standard
Solubility (in Water)	Clear, colorless solution
Loss on Drying	≤ 2.0%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.